Abstract
Objectives: To assess the in vitro activity of the FDA-approved antihelminthic drug pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis. Methods: A head-to-head comparison of a standard radiolabelled thymidine incorporation assay and the SYBR Green I-based fluorescence assay for determination of in vitro inhibition by pyrvinium and metronidazole was performed. Results: The 50% inhibitory concentration (IC50) for treatment of E. histolytica with pyrvinium was 4-5 μM for both assays compared with 1-2 μM for metronidazole. For pyrvinium treatment of G. intestinalis, an IC50 of ∼12 μM was determined by the radiolabelled thymidine assay alone, with maximum inhibition around 60%. In contrast, the IC50 for metronidazole treatment using this assay was ∼2 μM. Conclusions: Pyrvinium is a potential gut lumen agent for treatment of intestinal amoebiasis, but possibly not for giardiasis. SYBR Green I is an alternative screening method for E. histolytica, but not G. intestinalis.
Original language | English (US) |
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Pages (from-to) | 751-754 |
Number of pages | 4 |
Journal | Journal of Antimicrobial Chemotherapy |
Volume | 64 |
Issue number | 4 |
DOIs | |
State | Published - 2009 |
Keywords
- Drug
- Drug assay
- Protozoan
ASJC Scopus subject areas
- Pharmacology
- Microbiology (medical)
- Pharmacology (medical)
- Infectious Diseases