In utero MRI of mouse embryos

Jiangyang Zhang, Dan Wu, Daniel H. Turnbull

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Genetically engineered mouse models are used extensively as models of human development and developmental diseases. Conventional histological approaches are static and two-dimensional, and do not provide a full understanding of the dynamic, spatiotemporal changes in developing mouse embryos. Magnetic resonance imaging (MRI) offers a noninvasive and longitudinal approach for three-dimensional in utero imaging of normal and mutant mouse embryos. In this chapter, we describe MRI approaches that have been developed for imaging the living embryonic mouse brain and vasculature. Details are provided on the animal preparation and setup, MRI equipment, acquisition and reconstruction methods that have been found to be most useful for in utero MRI, including examples of applications to fetal mouse neuroimaging.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages285-296
Number of pages12
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1718
ISSN (Print)1064-3745

Keywords

  • Diffusion MRI
  • Diffusion weighted gradient and spin echo (DW-GRASE)
  • Field of excitation (FOE)
  • Fractional anisotropy (FA)
  • High-field MRI
  • Mn-enhanced MRI (MEMRI)
  • Phased array coil
  • Three-dimensional (3D)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Zhang, J., Wu, D., & Turnbull, D. H. (2018). In utero MRI of mouse embryos. In Methods in Molecular Biology (pp. 285-296). (Methods in Molecular Biology; Vol. 1718). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7531-0_17