In situ macrophage phenotypic transition is affected by altered cellular composition prior to acute sterile muscle injury

Andreas Patsalos, Attila Pap, Tamas Varga, Gyorgy Trencsenyi, Gerardo Alvarado Contreras, Ildiko Garai, Zoltan Papp, Balazs Dezso, Eva Pintye, Laszlo Nagy

Research output: Contribution to journalArticle

Abstract

Key points: The in situ phenotypic switch of macrophages is delayed in acute injury following irradiation. The combination of bone marrow transplantation and local muscle radiation protection allows for the identification of a myeloid cell contribution to tissue repair. PET-MRI allows monitoring of myeloid cell invasion and metabolism. Altered cellular composition prior to acute sterile injury affects the in situ phenotypic transition of invading myeloid cells to repair macrophages. There is reciprocal intercellular communication between local muscle cell compartments, such as PAX7 positive cells, and recruited macrophages during skeletal muscle regeneration. Abstract: Skeletal muscle regeneration is a complex interplay between various cell types including invading macrophages. Their recruitment to damaged tissues upon acute sterile injuries is necessary for clearance of necrotic debris and for coordination of tissue regeneration. This highly dynamic process is characterized by an in situ transition of infiltrating monocytes from an inflammatory (Ly6Chigh) to a repair (Ly6Clow) macrophage phenotype. The importance of the macrophage phenotypic shift and the cross-talk of the local muscle tissue with the infiltrating macrophages during tissue regeneration upon injury are not fully understood and their study lacks adequate methodology. Here, using an acute sterile skeletal muscle injury model combined with irradiation, bone marrow transplantation and in vivo imaging, we show that preserved muscle integrity and cell composition prior to the injury is necessary for the repair macrophage phenotypic transition and subsequently for proper and complete tissue regeneration. Importantly, by using a model of in vivo ablation of PAX7 positive cells, we show that this radiosensitive skeletal muscle progenitor pool contributes to macrophage phenotypic transition following acute sterile muscle injury. In addition, local muscle tissue radioprotection by lead shielding during irradiation preserves normal macrophage transition dynamics and subsequently muscle tissue regeneration. Taken together, our data suggest the existence of a more extensive and reciprocal cross-talk between muscle tissue compartments, including satellite cells, and infiltrating myeloid cells upon tissue damage. These interactions shape the macrophage in situ phenotypic shift, which is indispensable for normal muscle tissue repair dynamics. (Figure presented.).

Original languageEnglish (US)
Pages (from-to)5815-5842
Number of pages28
JournalJournal of Physiology
Volume595
Issue number17
DOIs
StatePublished - Sep 1 2017
Externally publishedYes

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Macrophages
Muscles
Wounds and Injuries
Regeneration
Myeloid Cells
Skeletal Muscle
Bone Marrow Transplantation
Muscle Cells
Radiation Protection
Monocytes
Phenotype

Keywords

  • bone marrow transplantation
  • inflammation
  • irradiation
  • macrophage polarization
  • muscle damage
  • radioprotection
  • regeneration
  • satellite cells

ASJC Scopus subject areas

  • Physiology

Cite this

In situ macrophage phenotypic transition is affected by altered cellular composition prior to acute sterile muscle injury. / Patsalos, Andreas; Pap, Attila; Varga, Tamas; Trencsenyi, Gyorgy; Contreras, Gerardo Alvarado; Garai, Ildiko; Papp, Zoltan; Dezso, Balazs; Pintye, Eva; Nagy, Laszlo.

In: Journal of Physiology, Vol. 595, No. 17, 01.09.2017, p. 5815-5842.

Research output: Contribution to journalArticle

Patsalos, A, Pap, A, Varga, T, Trencsenyi, G, Contreras, GA, Garai, I, Papp, Z, Dezso, B, Pintye, E & Nagy, L 2017, 'In situ macrophage phenotypic transition is affected by altered cellular composition prior to acute sterile muscle injury', Journal of Physiology, vol. 595, no. 17, pp. 5815-5842. https://doi.org/10.1113/JP274361
Patsalos, Andreas ; Pap, Attila ; Varga, Tamas ; Trencsenyi, Gyorgy ; Contreras, Gerardo Alvarado ; Garai, Ildiko ; Papp, Zoltan ; Dezso, Balazs ; Pintye, Eva ; Nagy, Laszlo. / In situ macrophage phenotypic transition is affected by altered cellular composition prior to acute sterile muscle injury. In: Journal of Physiology. 2017 ; Vol. 595, No. 17. pp. 5815-5842.
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