In situ hybridization to the crithidia fasciculata kinetoplast reveals two antipodal sites involved in kinetoplast DNA replication

Martin Ferguson, Al F. Torri, David C. Ward, Paul T. Englund

Research output: Contribution to journalArticle

Abstract

Kinetoplast DNA is a network of interlocked minicircles and maxicircles. In situ hybridization, using probes detected by digital fluorescence microscopy, has clarified the in vivo structure and replication mechanism of the network. The probe recognizes only nicked minicircles. Hybridization reveals prereplication kinetoplasts (with closed minicircles), donutshaped replicating kinetoplasts (with nicked minicircles on the periphery and closed minicircles in the center), and postreplication kinetoplasts (with nicked minicircles). Replicating kinetoplasts are associated with two peripheral structures containing free minicircle replication intermediates and DNA polymerase. Replication may involve release of closed minicircles from the center of the kinetoplast and their migration to the peripheral structures, replication of the free minicircles therein, and then peripheral reattachment of the progeny minicircles to the kinetoplast.

Original languageEnglish (US)
Pages (from-to)621-629
Number of pages9
JournalCell
Volume70
Issue number4
DOIs
Publication statusPublished - Aug 21 1992

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ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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