In situ hybridization approach to study mRNA expression and distribution in cochlear frozen sections

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In situ hybridization is well suited to obtaining specific topological information on gene transcripts and thereby to relating such observations to a particular function. In spite of the technical and practical difficulties, the application of molecular biological techniques such as in situ hybridization to the cochlea can provide important insights. However, the rarity of gene products (mRNA and proteins) in the cochlea and its fragile structure require the refinement and adaptation of in situ hybridization methods. The present chapter provides a detailed in situ hybridization protocol adapted to frozen tissue sections collected from adult and neonatal stages of the vertebrate cochlea.

Original languageEnglish (US)
Title of host publicationAuditory and Vestibular Research
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages31-46
Number of pages16
ISBN (Print)9781934115626
DOIs
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
Volume493
ISSN (Print)1064-3745

Keywords

  • -UTP
  • Autoradiography
  • Cochlea
  • Digoxigenin-UTP
  • Ear
  • Gene expression
  • In situ hybridization
  • Riboprobes
  • Vestibule

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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