In situ detection of degraded and denatured collagen via triple helical hybridization: New tool in histopathology

Yang Li, S. Michael Yu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Degraded and denatured collagens are useful markers for physiological events (e.g., bone formation and aging) and pathologic conditions (e.g., cancer, arthritis, and fibrosis). Here we describe histological staining of such collagens using fluorescent collagen hybridizing peptide that can specifically bind to collagen strands by folding into triple helix. The method can report the amount of denatured collagen and/or collagen remodeling activity in tissues via localized fluorescence intensity and can be used in conjunction with conventional staining agents. The collagen hybridizing peptide probes can be used across species and collagen types, providing a versatile tool not only for pathology and developmental biology but also histology-based disease diagnosis, staging, and therapeutic screening.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages135-144
Number of pages10
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1944
ISSN (Print)1064-3745

Keywords

  • Bone resorption
  • Collagen fiber
  • Collagen remodeling
  • Collagenase
  • Immunostaining
  • Matrix metalloproteinase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Li, Y., & Yu, S. M. (2019). In situ detection of degraded and denatured collagen via triple helical hybridization: New tool in histopathology. In Methods in Molecular Biology (pp. 135-144). (Methods in Molecular Biology; Vol. 1944). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9095-5_10