In indirect flight muscles Drosophila projectin has a short PEVK domain, and its NH2-terminus is embedded at the Z-band

Agnes Ayme-Southgate, Judith Saide, Richard Southgate, Christophe Bounaix, Anthony Cammarato, Sunita Patel, Catherine Wussler

Research output: Contribution to journalArticlepeer-review

Abstract

Insect indirect flight muscles (IFM) contain a third filament system made up of elastic connecting or C-filaments. The giant protein projectin is the main, if not the only, component of these structures. In this study we found that projectin is oriented within the IFM sarcomere with its NH 2-terminus embedded in the Z-bands. We demonstrate that this protein has an elastic region that can be detected by the movement of specific epitopes following stretch. One possible elastic region is the PEVK-like domain located close to the NH2-terminus. The amino acid length of this region is short, and 52% of its residues are P, E, V or K. We propose a model in which projectin extends from the Z-band to the lateral borders of the A-band. The PEVK-like domain and a series of Ig domains spanning the intervening I-band may provide the elastic properties of projectin.

Original languageEnglish (US)
Pages (from-to)467-477
Number of pages11
JournalJournal of Muscle Research and Cell Motility
Volume26
Issue number6-8
DOIs
StatePublished - Dec 1 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Cell Biology

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