In-frame linker insertion mutagenesis of yeast transposon Ty1: mutations, transposition and dominance

G. M. Monokian, L. T. Braiterman, J. D. Boeke

Research output: Contribution to journalArticle

Abstract

A plasmid bearing a GAL1::Tyl fusion that is competent to transpose was mutagenized by insertion of oligodeoxyribo-nucleotides that precisely introduce four or five codons semirandomly throughout the plasmid. Approximately one quarter of these resulted in inactivation of transposition; these include inactivating insertions in both the TYA and TYB genes, corresponding to retroviral gag and pol genes. Examples of transposition-inactivating mutations map within each of the known or proposed functional domains of TYB, suggesting that these are all required for retrotransposition. All of the transposition-inactivating mutations were found to be recessive with the exception of a single mutation in TYA. The remaining mutations have slightly deleterious to no effect on Ty1 transposition.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalGene
Volume139
Issue number1
DOIs
StatePublished - Feb 11 1994

Fingerprint

Retroelements
Insertional Mutagenesis
Yeasts
Mutation
Plasmids
pol Genes
gag Genes
Codon
Nucleotides
Genes

Keywords

  • codon insertion mutations
  • Retrotransposons
  • vSaccharomyces cerevisiae

ASJC Scopus subject areas

  • Genetics

Cite this

In-frame linker insertion mutagenesis of yeast transposon Ty1 : mutations, transposition and dominance. / Monokian, G. M.; Braiterman, L. T.; Boeke, J. D.

In: Gene, Vol. 139, No. 1, 11.02.1994, p. 9-18.

Research output: Contribution to journalArticle

Monokian, G. M. ; Braiterman, L. T. ; Boeke, J. D. / In-frame linker insertion mutagenesis of yeast transposon Ty1 : mutations, transposition and dominance. In: Gene. 1994 ; Vol. 139, No. 1. pp. 9-18.
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