In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas

Diana Bell, Pascale Chomarat, Denise Broyles, George Netto, Ghada Moumneh Harb, Serge Lebecque, Jenny Valladeau, Jean Davoust, Karolina A. Palucka, Jacques Banchereau

Research output: Contribution to journalArticle

Abstract

We have analyzed the presence of immature and mature dendritic cells (DCs) within adenocarcinoma of the breast using immunohistochemistry. Immature DCs were defined by expression of CD1a-, Langerin-, and intracellular major histocompatibility complex class II-rich vesicles. Mature DCs were defined by expression of CD83 and DC-Lamp. Breast carcinoma cells were defined by morphology and/or cytokeratin expression. We demonstrate two levels of heterogeneity of DCs infiltrating breast carcinoma tissue: (a) immature CD1a+ DCs, mostly of the Langerhans cell type (Langerin+), were retained within the tumor bed in 32/32 samples and (b) mature DCs, CD83+DC- Lamp+, present in 20/32 samples, are confined to peritumoral areas. The high numbers of immature DCs found in the tumor may be best explained by high levels of macrophage inflammatory protein 3α expression by virtually all tumor cells. Confirming the immature/mature DC compartmentalization pattern, in vitro-generated immature DCs adhere to the tumor cells, whereas mature DCs adhere selectively to peritumoral areas. In some cases, T cells are clustering around the mature DCs in peritumoral areas, thus resembling the DC-T cell clusters of secondary lymphoid organs, which are characteristic of ongoing immune reactions.

Original languageEnglish (US)
Pages (from-to)1417-1425
Number of pages9
JournalJournal of Experimental Medicine
Volume190
Issue number10
DOIs
StatePublished - Nov 15 1999
Externally publishedYes

Fingerprint

Dendritic Cells
Breast Neoplasms
Neoplasms
T-Lymphocytes
Macrophage Inflammatory Proteins
Langerhans Cells
Keratins
Major Histocompatibility Complex
Cluster Analysis
Adenocarcinoma
Breast
Immunohistochemistry

Keywords

  • Breast cancer
  • Chemokines
  • Dendritic cells
  • MHC class II
  • Tumor immunity

ASJC Scopus subject areas

  • Immunology

Cite this

In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas. / Bell, Diana; Chomarat, Pascale; Broyles, Denise; Netto, George; Harb, Ghada Moumneh; Lebecque, Serge; Valladeau, Jenny; Davoust, Jean; Palucka, Karolina A.; Banchereau, Jacques.

In: Journal of Experimental Medicine, Vol. 190, No. 10, 15.11.1999, p. 1417-1425.

Research output: Contribution to journalArticle

Bell, D, Chomarat, P, Broyles, D, Netto, G, Harb, GM, Lebecque, S, Valladeau, J, Davoust, J, Palucka, KA & Banchereau, J 1999, 'In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas', Journal of Experimental Medicine, vol. 190, no. 10, pp. 1417-1425. https://doi.org/10.1084/jem.190.10.1417
Bell, Diana ; Chomarat, Pascale ; Broyles, Denise ; Netto, George ; Harb, Ghada Moumneh ; Lebecque, Serge ; Valladeau, Jenny ; Davoust, Jean ; Palucka, Karolina A. ; Banchereau, Jacques. / In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas. In: Journal of Experimental Medicine. 1999 ; Vol. 190, No. 10. pp. 1417-1425.
@article{db544d02bad74d4fab8e62cc5ed03b9a,
title = "In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas",
abstract = "We have analyzed the presence of immature and mature dendritic cells (DCs) within adenocarcinoma of the breast using immunohistochemistry. Immature DCs were defined by expression of CD1a-, Langerin-, and intracellular major histocompatibility complex class II-rich vesicles. Mature DCs were defined by expression of CD83 and DC-Lamp. Breast carcinoma cells were defined by morphology and/or cytokeratin expression. We demonstrate two levels of heterogeneity of DCs infiltrating breast carcinoma tissue: (a) immature CD1a+ DCs, mostly of the Langerhans cell type (Langerin+), were retained within the tumor bed in 32/32 samples and (b) mature DCs, CD83+DC- Lamp+, present in 20/32 samples, are confined to peritumoral areas. The high numbers of immature DCs found in the tumor may be best explained by high levels of macrophage inflammatory protein 3α expression by virtually all tumor cells. Confirming the immature/mature DC compartmentalization pattern, in vitro-generated immature DCs adhere to the tumor cells, whereas mature DCs adhere selectively to peritumoral areas. In some cases, T cells are clustering around the mature DCs in peritumoral areas, thus resembling the DC-T cell clusters of secondary lymphoid organs, which are characteristic of ongoing immune reactions.",
keywords = "Breast cancer, Chemokines, Dendritic cells, MHC class II, Tumor immunity",
author = "Diana Bell and Pascale Chomarat and Denise Broyles and George Netto and Harb, {Ghada Moumneh} and Serge Lebecque and Jenny Valladeau and Jean Davoust and Palucka, {Karolina A.} and Jacques Banchereau",
year = "1999",
month = "11",
day = "15",
doi = "10.1084/jem.190.10.1417",
language = "English (US)",
volume = "190",
pages = "1417--1425",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "10",

}

TY - JOUR

T1 - In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas

AU - Bell, Diana

AU - Chomarat, Pascale

AU - Broyles, Denise

AU - Netto, George

AU - Harb, Ghada Moumneh

AU - Lebecque, Serge

AU - Valladeau, Jenny

AU - Davoust, Jean

AU - Palucka, Karolina A.

AU - Banchereau, Jacques

PY - 1999/11/15

Y1 - 1999/11/15

N2 - We have analyzed the presence of immature and mature dendritic cells (DCs) within adenocarcinoma of the breast using immunohistochemistry. Immature DCs were defined by expression of CD1a-, Langerin-, and intracellular major histocompatibility complex class II-rich vesicles. Mature DCs were defined by expression of CD83 and DC-Lamp. Breast carcinoma cells were defined by morphology and/or cytokeratin expression. We demonstrate two levels of heterogeneity of DCs infiltrating breast carcinoma tissue: (a) immature CD1a+ DCs, mostly of the Langerhans cell type (Langerin+), were retained within the tumor bed in 32/32 samples and (b) mature DCs, CD83+DC- Lamp+, present in 20/32 samples, are confined to peritumoral areas. The high numbers of immature DCs found in the tumor may be best explained by high levels of macrophage inflammatory protein 3α expression by virtually all tumor cells. Confirming the immature/mature DC compartmentalization pattern, in vitro-generated immature DCs adhere to the tumor cells, whereas mature DCs adhere selectively to peritumoral areas. In some cases, T cells are clustering around the mature DCs in peritumoral areas, thus resembling the DC-T cell clusters of secondary lymphoid organs, which are characteristic of ongoing immune reactions.

AB - We have analyzed the presence of immature and mature dendritic cells (DCs) within adenocarcinoma of the breast using immunohistochemistry. Immature DCs were defined by expression of CD1a-, Langerin-, and intracellular major histocompatibility complex class II-rich vesicles. Mature DCs were defined by expression of CD83 and DC-Lamp. Breast carcinoma cells were defined by morphology and/or cytokeratin expression. We demonstrate two levels of heterogeneity of DCs infiltrating breast carcinoma tissue: (a) immature CD1a+ DCs, mostly of the Langerhans cell type (Langerin+), were retained within the tumor bed in 32/32 samples and (b) mature DCs, CD83+DC- Lamp+, present in 20/32 samples, are confined to peritumoral areas. The high numbers of immature DCs found in the tumor may be best explained by high levels of macrophage inflammatory protein 3α expression by virtually all tumor cells. Confirming the immature/mature DC compartmentalization pattern, in vitro-generated immature DCs adhere to the tumor cells, whereas mature DCs adhere selectively to peritumoral areas. In some cases, T cells are clustering around the mature DCs in peritumoral areas, thus resembling the DC-T cell clusters of secondary lymphoid organs, which are characteristic of ongoing immune reactions.

KW - Breast cancer

KW - Chemokines

KW - Dendritic cells

KW - MHC class II

KW - Tumor immunity

UR - http://www.scopus.com/inward/record.url?scp=0033571785&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033571785&partnerID=8YFLogxK

U2 - 10.1084/jem.190.10.1417

DO - 10.1084/jem.190.10.1417

M3 - Article

VL - 190

SP - 1417

EP - 1425

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 10

ER -