In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas

Diana Bell, Pascale Chomarat, Denise Broyles, George Netto, Ghada Moumneh Harb, Serge Lebecque, Jenny Valladeau, Jean Davoust, Karolina A. Palucka, Jacques Banchereau

Research output: Contribution to journalArticlepeer-review

Abstract

We have analyzed the presence of immature and mature dendritic cells (DCs) within adenocarcinoma of the breast using immunohistochemistry. Immature DCs were defined by expression of CD1a-, Langerin-, and intracellular major histocompatibility complex class II-rich vesicles. Mature DCs were defined by expression of CD83 and DC-Lamp. Breast carcinoma cells were defined by morphology and/or cytokeratin expression. We demonstrate two levels of heterogeneity of DCs infiltrating breast carcinoma tissue: (a) immature CD1a+ DCs, mostly of the Langerhans cell type (Langerin+), were retained within the tumor bed in 32/32 samples and (b) mature DCs, CD83+DC- Lamp+, present in 20/32 samples, are confined to peritumoral areas. The high numbers of immature DCs found in the tumor may be best explained by high levels of macrophage inflammatory protein 3α expression by virtually all tumor cells. Confirming the immature/mature DC compartmentalization pattern, in vitro-generated immature DCs adhere to the tumor cells, whereas mature DCs adhere selectively to peritumoral areas. In some cases, T cells are clustering around the mature DCs in peritumoral areas, thus resembling the DC-T cell clusters of secondary lymphoid organs, which are characteristic of ongoing immune reactions.

Original languageEnglish (US)
Pages (from-to)1417-1425
Number of pages9
JournalJournal of Experimental Medicine
Volume190
Issue number10
DOIs
StatePublished - Nov 15 1999

Keywords

  • Breast cancer
  • Chemokines
  • Dendritic cells
  • MHC class II
  • Tumor immunity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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