Imputation of Variants from the 1000 Genomes Project Modestly Improves Known Associations and Can Identify Low-frequency Variant - Phenotype Associations Undetected by HapMap Based Imputation

Andrew R. Wood, John R B Perry, Toshiko Tanaka, Dena G. Hernandez, Hou Feng Zheng, David Melzer, J. Raphael Gibbs, Michael A. Nalls, Michael N. Weedon, Tim D. Spector, J. Brent Richards, Stefania Bandinelli, Luigi Ferrucci, Andrew B. Singleton, Timothy M. Frayling

Research output: Contribution to journalArticlepeer-review

Abstract

Genome-wide association (GWA) studies have been limited by the reliance on common variants present on microarrays or imputable from the HapMap Project data. More recently, the completion of the 1000 Genomes Project has provided variant and haplotype information for several million variants derived from sequencing over 1,000 individuals. To help understand the extent to which more variants (including low frequency (1% ≤ MAF -8 based on HapMap and 1000 Genomes imputation, respectively, and 9 and 11 that reached a stricter, likely conservative, threshold of P-11 respectively. Imputation of 1000 Genomes genotype data modestly improved the strength of known associations. Of 20 associations detected at P-8 in both analyses (17 of which represent well replicated signals in the NHGRI catalogue), six were captured by the same index SNP, five were nominally more strongly associated in 1000 Genomes imputed data and one was nominally more strongly associated in HapMap imputed data. We also detected an association between a low frequency variant and phenotype that was previously missed by HapMap based imputation approaches. An association between rs112635299 and alpha-1 globulin near the SERPINA gene represented the known association between rs28929474 (MAF = 0.007) and alpha1-antitrypsin that predisposes to emphysema (P = 2.5×10-12). Our data provide important proof of principle that 1000 Genomes imputation will detect novel, low frequency-large effect associations.

Original languageEnglish (US)
Article numbere64343
JournalPLoS One
Volume8
Issue number5
DOIs
StatePublished - May 16 2013
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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