Improving UV resistance and virulence of Beauveria bassiana by genetic engineering with an exogenous tyrosinase gene

Yanfang Shang, Zhibing Duan, Wei Huang, Qiang Gao, Chengshu Wang

Research output: Contribution to journalArticle

Abstract

Insect pathogenic fungi like Beauveria bassiana have been developed as environmentally friendly biocontrol agents against arthropod pests. However, restrictive environmental factors, including solar ultraviolet (UV) radiation frequently lead to inconsistent field performance. To improve resistance to UV damage, we used Agrobacterium-mediated transformation to engineer B. bassiana with an exogenous tyrosinase gene. The results showed that the mitotically stable transformants produced larger amounts of yellowish pigments than the wild-type strain, and these imparted significantly increased UV-resistance. The virulence of the transgenic isolate was also significantly increased against the silkworm Bombyx mori and the mealworm Tenebrio molitor. This study demonstrated that genetic engineering of B. bassiana with a tyrosinase gene is an effective way to improve fungal tolerance against UV damage.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalJournal of Invertebrate Pathology
Volume109
Issue number1
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Keywords

  • Beauveria bassiana
  • Genetic engineering
  • Melanin
  • Tyrosinase
  • UV-resistance
  • Virulence

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Fingerprint Dive into the research topics of 'Improving UV resistance and virulence of Beauveria bassiana by genetic engineering with an exogenous tyrosinase gene'. Together they form a unique fingerprint.

  • Cite this