Improving the sensitivity of blood culture for Streptococcus pneumoniae

Samir Saha, Gary Darmstadt, Aliya Naheed, Shams Arifeen, Maksuda Islam, Kaniz Fatima, Robert Breiman, David Sack, Davidson Hamer

Research output: Contribution to journalArticle

Abstract

Isolation of Streptococcus pneumoniae is jeopardized by low sensitivity of blood culture, autolysis and contamination with fast-growing organism(s). We performed an immunochromatographic (ICT) test for S. pneumoniae on chocolatized blood culture bottles and also sub-cultured contaminated bottles on a selective medium, thus identifying an additional eight and three cases, respectively, and improving the detection of pneumococcus by 23% (48% vs. 59%). Prescreening of culture bottles in a blinded fashion could rationalize the use of ICT with ~99% accuracy. These two approaches can aid microbiology laboratories in resource-poor countries to substantially improve rates of detection of S. pneumoniae.

LanguageEnglish (US)
Article numberfmq070
Pages192-196
Number of pages5
JournalJournal of Tropical Pediatrics
Volume57
Issue number3
DOIs
StatePublished - Jun 2011

Fingerprint

Streptococcus pneumoniae
Autolysis
Microbiology
Blood Culture

Keywords

  • Autolysin
  • Blood culture
  • Immunochromatographic test
  • Pneumococcus

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Infectious Diseases

Cite this

Saha, S., Darmstadt, G., Naheed, A., Arifeen, S., Islam, M., Fatima, K., ... Hamer, D. (2011). Improving the sensitivity of blood culture for Streptococcus pneumoniae. Journal of Tropical Pediatrics, 57(3), 192-196. [fmq070]. DOI: 10.1093/tropej/fmq070

Improving the sensitivity of blood culture for Streptococcus pneumoniae. / Saha, Samir; Darmstadt, Gary; Naheed, Aliya; Arifeen, Shams; Islam, Maksuda; Fatima, Kaniz; Breiman, Robert; Sack, David; Hamer, Davidson.

In: Journal of Tropical Pediatrics, Vol. 57, No. 3, fmq070, 06.2011, p. 192-196.

Research output: Contribution to journalArticle

Saha, S, Darmstadt, G, Naheed, A, Arifeen, S, Islam, M, Fatima, K, Breiman, R, Sack, D & Hamer, D 2011, 'Improving the sensitivity of blood culture for Streptococcus pneumoniae' Journal of Tropical Pediatrics, vol. 57, no. 3, fmq070, pp. 192-196. DOI: 10.1093/tropej/fmq070
Saha S, Darmstadt G, Naheed A, Arifeen S, Islam M, Fatima K et al. Improving the sensitivity of blood culture for Streptococcus pneumoniae. Journal of Tropical Pediatrics. 2011 Jun;57(3):192-196. fmq070. Available from, DOI: 10.1093/tropej/fmq070
Saha, Samir ; Darmstadt, Gary ; Naheed, Aliya ; Arifeen, Shams ; Islam, Maksuda ; Fatima, Kaniz ; Breiman, Robert ; Sack, David ; Hamer, Davidson. / Improving the sensitivity of blood culture for Streptococcus pneumoniae. In: Journal of Tropical Pediatrics. 2011 ; Vol. 57, No. 3. pp. 192-196
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