Improving the resolving power in a miniature MALDI TOP mass spectrometer

Robert D. English; Robert J. Cotter

Improving the resolving power in a miniature MALDI TOP mass spectrometer

School of Medicine

Research output: Contribution to conference › Paper › peer-review

Abstract

Mass correlated acceleration (MCA) was combined with a shorter time-of-flight (TOF) analyzer in a MALDI TOF mass spectrometer to assess the performance of MCA in a miniature instrument. Instrumental parameters such as TOF dimensions and delay time settings were optimized to achieve optimum ion focusing. Mass spectra were collected using a custom MALDI instrument with a pulsed nitrogen laser. The results show that compared to pulsed extraction (PE), MCA supplied better focusing across a wider mass range for a protein tryptic digestion and a peptide mixture.

Original language	English (US)
Pages	863-864
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

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title = "Improving the resolving power in a miniature MALDI TOP mass spectrometer",

abstract = "Mass correlated acceleration (MCA) was combined with a shorter time-of-flight (TOF) analyzer in a MALDI TOF mass spectrometer to assess the performance of MCA in a miniature instrument. Instrumental parameters such as TOF dimensions and delay time settings were optimized to achieve optimum ion focusing. Mass spectra were collected using a custom MALDI instrument with a pulsed nitrogen laser. The results show that compared to pulsed extraction (PE), MCA supplied better focusing across a wider mass range for a protein tryptic digestion and a peptide mixture.",

author = "English, {Robert D.} and Cotter, {Robert J.}",

year = "2002",

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language = "English (US)",

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N2 - Mass correlated acceleration (MCA) was combined with a shorter time-of-flight (TOF) analyzer in a MALDI TOF mass spectrometer to assess the performance of MCA in a miniature instrument. Instrumental parameters such as TOF dimensions and delay time settings were optimized to achieve optimum ion focusing. Mass spectra were collected using a custom MALDI instrument with a pulsed nitrogen laser. The results show that compared to pulsed extraction (PE), MCA supplied better focusing across a wider mass range for a protein tryptic digestion and a peptide mixture.

AB - Mass correlated acceleration (MCA) was combined with a shorter time-of-flight (TOF) analyzer in a MALDI TOF mass spectrometer to assess the performance of MCA in a miniature instrument. Instrumental parameters such as TOF dimensions and delay time settings were optimized to achieve optimum ion focusing. Mass spectra were collected using a custom MALDI instrument with a pulsed nitrogen laser. The results show that compared to pulsed extraction (PE), MCA supplied better focusing across a wider mass range for a protein tryptic digestion and a peptide mixture.

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