Improving efficacy of interleukin-12-transfected dendritic cells injected into murine colon cancer with anti-CD137 monoclonal antibodies and alloantigens

Iñigo Tirapui, Ainhoa Arina, Guillermo Mazzolinii, Marina Duarte, Carlos Alfaroi, Esperanza Feijoo, Cheng Qian, Lieping Chen, Jesus Prieto, Ignacio Melero

Research output: Contribution to journalArticle

Abstract

Intralesional administration of cultured dendritic cells (DCs) engineered to produce IL-12 by in vitro infection with recombinant adenovirus frequently displays eradicating efficacy against established subcutaneous tumors derived from the CT26 murine colon carcinoma cell line. The elicited response is mainly mediated by cytolitic T lymphocytes. In order to search for strategies that would enhance the efficacy of the therapeutic procedure against less immunogenic tumors, we moved onto malignancies derived from the inoculation of MC38 colon cancer cells that are less prone to undergo complete regression upon a single intratumoral injection of IL-12-secreting DCs. In this model, we found that repeated injections of such DCs, as opposed to a single injection, achieved better efficacy against both the injected and a distantly implanted tumor; that the use of semiallogeneic DCs that are mismatched in one MHC haplotype with the tumor host showed slightly better efficacy; and that the combination of this treatment with systemic injections of immunostimulatory anti-CD137 (4-1BB) monoclonal antibody achieved potent combined effects that correlated with the antitumor immune response measured in IFN-γ ELISPOT assays. The elicited systemic immune response eradicates concomitant untreated lesions in most cases. Curative efficacy was also found against some tumors established for 2 weeks when these strategies were used in combination. These are preclinical pieces of evidence to be considered in order to enhance the therapeutic benefit of a strategy that is currently being tested in clinical trials.

Original languageEnglish (US)
Pages (from-to)51-60
Number of pages10
JournalInternational Journal of Cancer
Volume110
Issue number1
DOIs
Publication statusPublished - May 20 2004
Externally publishedYes

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Keywords

  • Alloantigen
  • CD137
  • Dendritic cells
  • IL-12
  • Tumor immunotherapy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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