Improving DNA vaccine potency by linking Marek's disease virus type 1 VP22 to an antigen

Chien Fu Hung, Liangmei He, Jeremy Juang, Tzyy Jye Lin, Morris Ling, T. C. Wu

Research output: Contribution to journalArticle

Abstract

We have previously employed an intercellular spreading strategy using herpes simplex virus type 1 (HSV-1) VP22 protein to enhance DNA vaccine potency because DNA vaccines lack the intrinsic ability to amplify in cells. Recently, studies have demonstrated that the protein encoded by UL49 of Marek's disease virus type 1 (MDV-1) exhibits some degree of homology to the HSV-1 VP22 protein and features the property of intercellular transport. We therefore generated a DNA vaccine encoding MDV-1 VP22 linked to a model antigen, human papillomavirus type 16 E7. We demonstrated that compared with mice vaccinated with DNA encoding wild-type E7, mice vaccinated with MDV-1 VP22/E7 DNA exhibited a significant increase in number of gamma-interferon-secreting, E7-specific CD8+-T-cell precursors as well as stronger tumor prevention and treatment effects. Furthermore, our data indicated that the antitumor effect was CD8 dependent. These results suggested that the development of vaccines encoding VP22 fused to a target antigen might be a promising strategy for improving DNA vaccine potency.

Original languageEnglish (US)
Pages (from-to)2676-2682
Number of pages7
JournalJournal of virology
Volume76
Issue number6
DOIs
StatePublished - Mar 11 2002

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Improving DNA vaccine potency by linking Marek's disease virus type 1 VP22 to an antigen'. Together they form a unique fingerprint.

  • Cite this