TY - JOUR
T1 - Improvements in two-dimensional gel electrophoresis by utilizing a low cost "in-house" neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system
AU - Graham, David R.M.
AU - Garnham, Christopher P.
AU - Fu, Qin
AU - Robbins, Jeffrey
AU - Van Eyk, Jennifer E.
PY - 2005/6
Y1 - 2005/6
N2 - Two-dimensional gel electrophoresis (2-DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis ((SDS-PAGE)/(Bis (2-hydroxyethyl) imino-tris (hydroxymethyl) methane-HCl, or Bis-Tris)) have greatly improved this technique, but cost and limited sizes restrict their applications. An "in-house" system is presented, resulting in better resolution, separation, and new spot visualization and improved resolution when compared to Tris-HCl gels. Their utility is demonstrated using albumin-depleted serum samples, rabbit heart left ventricle, and human immunodeficiency virus type 1 (HIV-1).
AB - Two-dimensional gel electrophoresis (2-DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis ((SDS-PAGE)/(Bis (2-hydroxyethyl) imino-tris (hydroxymethyl) methane-HCl, or Bis-Tris)) have greatly improved this technique, but cost and limited sizes restrict their applications. An "in-house" system is presented, resulting in better resolution, separation, and new spot visualization and improved resolution when compared to Tris-HCl gels. Their utility is demonstrated using albumin-depleted serum samples, rabbit heart left ventricle, and human immunodeficiency virus type 1 (HIV-1).
KW - Bis-Tris
KW - Casdiac
KW - Human immunodeficiency virus type 1
KW - Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=20644455953&partnerID=8YFLogxK
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U2 - 10.1002/pmic.200401249
DO - 10.1002/pmic.200401249
M3 - Article
C2 - 15887185
AN - SCOPUS:20644455953
VL - 5
SP - 2309
EP - 2314
JO - Proteomics
JF - Proteomics
SN - 1615-9853
IS - 9
ER -