Improvements in two-dimensional gel electrophoresis by utilizing a low cost "in-house" neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system

David Graham, Christopher P. Garnham, Qin Fu, Jeffrey Robbins, Jennifer E. Van Eyk

Research output: Contribution to journalArticle

Abstract

Two-dimensional gel electrophoresis (2-DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis ((SDS-PAGE)/(Bis (2-hydroxyethyl) imino-tris (hydroxymethyl) methane-HCl, or Bis-Tris)) have greatly improved this technique, but cost and limited sizes restrict their applications. An "in-house" system is presented, resulting in better resolution, separation, and new spot visualization and improved resolution when compared to Tris-HCl gels. Their utility is demonstrated using albumin-depleted serum samples, rabbit heart left ventricle, and human immunodeficiency virus type 1 (HIV-1).

Original languageEnglish (US)
Pages (from-to)2309-2314
Number of pages6
JournalProteomics
Volume5
Issue number9
DOIs
StatePublished - Jun 2005

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Keywords

  • Bis-Tris
  • Casdiac
  • Human immunodeficiency virus type 1
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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