Improvements in two-dimensional gel electrophoresis by utilizing a low cost "in-house" neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system

David Graham; Christopher P. Garnham; Qin Fu; Jeffrey Robbins; Jennifer E. Van Eyk

doi:10.1002/pmic.200401249

Improvements in two-dimensional gel electrophoresis by utilizing a low cost "in-house" neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system

David Graham, Christopher P. Garnham, Qin Fu, Jeffrey Robbins, Jennifer E. Van Eyk

School of Medicine

Research output: Contribution to journal › Article

Abstract

Two-dimensional gel electrophoresis (2-DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis ((SDS-PAGE)/(Bis (2-hydroxyethyl) imino-tris (hydroxymethyl) methane-HCl, or Bis-Tris)) have greatly improved this technique, but cost and limited sizes restrict their applications. An "in-house" system is presented, resulting in better resolution, separation, and new spot visualization and improved resolution when compared to Tris-HCl gels. Their utility is demonstrated using albumin-depleted serum samples, rabbit heart left ventricle, and human immunodeficiency virus type 1 (HIV-1).

Original language	English (US)
Pages (from-to)	2309-2314
Number of pages	6
Journal	Proteomics
Volume	5
Issue number	9
DOIs	https://doi.org/10.1002/pmic.200401249
State	Published - Jun 2005

Fingerprint

Keywords

Bis-Tris
Casdiac
Human immunodeficiency virus type 1
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Graham, D., Garnham, C. P., Fu, Q., Robbins, J., & Van Eyk, J. E. (2005). Improvements in two-dimensional gel electrophoresis by utilizing a low cost "in-house" neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system. Proteomics, 5(9), 2309-2314. https://doi.org/10.1002/pmic.200401249

@article{f956215c0f194537bea97c4fcaa57c67,

title = "Improvements in two-dimensional gel electrophoresis by utilizing a low cost {"}in-house{"} neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system",

abstract = "Two-dimensional gel electrophoresis (2-DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis ((SDS-PAGE)/(Bis (2-hydroxyethyl) imino-tris (hydroxymethyl) methane-HCl, or Bis-Tris)) have greatly improved this technique, but cost and limited sizes restrict their applications. An {"}in-house{"} system is presented, resulting in better resolution, separation, and new spot visualization and improved resolution when compared to Tris-HCl gels. Their utility is demonstrated using albumin-depleted serum samples, rabbit heart left ventricle, and human immunodeficiency virus type 1 (HIV-1).",

keywords = "Bis-Tris, Casdiac, Human immunodeficiency virus type 1, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis",

author = "David Graham and Garnham, {Christopher P.} and Qin Fu and Jeffrey Robbins and {Van Eyk}, {Jennifer E.}",

year = "2005",

month = jun

doi = "10.1002/pmic.200401249",

language = "English (US)",

volume = "5",

pages = "2309--2314",

journal = "Proteomics",

issn = "1615-9853",

publisher = "Wiley-VCH Verlag",

number = "9",

}

TY - JOUR

T1 - Improvements in two-dimensional gel electrophoresis by utilizing a low cost "in-house" neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system

AU - Graham, David

AU - Garnham, Christopher P.

AU - Fu, Qin

AU - Robbins, Jeffrey

AU - Van Eyk, Jennifer E.

PY - 2005/6

Y1 - 2005/6

N2 - Two-dimensional gel electrophoresis (2-DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis ((SDS-PAGE)/(Bis (2-hydroxyethyl) imino-tris (hydroxymethyl) methane-HCl, or Bis-Tris)) have greatly improved this technique, but cost and limited sizes restrict their applications. An "in-house" system is presented, resulting in better resolution, separation, and new spot visualization and improved resolution when compared to Tris-HCl gels. Their utility is demonstrated using albumin-depleted serum samples, rabbit heart left ventricle, and human immunodeficiency virus type 1 (HIV-1).

AB - Two-dimensional gel electrophoresis (2-DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis ((SDS-PAGE)/(Bis (2-hydroxyethyl) imino-tris (hydroxymethyl) methane-HCl, or Bis-Tris)) have greatly improved this technique, but cost and limited sizes restrict their applications. An "in-house" system is presented, resulting in better resolution, separation, and new spot visualization and improved resolution when compared to Tris-HCl gels. Their utility is demonstrated using albumin-depleted serum samples, rabbit heart left ventricle, and human immunodeficiency virus type 1 (HIV-1).

KW - Bis-Tris

KW - Casdiac

KW - Human immunodeficiency virus type 1

KW - Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=20644455953&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20644455953&partnerID=8YFLogxK

U2 - 10.1002/pmic.200401249

DO - 10.1002/pmic.200401249

M3 - Article

C2 - 15887185

AN - SCOPUS:20644455953

VL - 5

SP - 2309

EP - 2314

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 9

ER -

Access to Document

10.1002/pmic.200401249