Improvements in the RT-PCR detection of enteric viruses in environmental samples

K. J. Schwab, F. H. Neill, M. K. Estes, R. L. Atmar

Research output: Contribution to journalConference articlepeer-review

Abstract

The current methods for the detection of nucleic acid from enteric viruses in environmental samples usually involve extensive concentration and purification of target viruses followed by RT-PCR amplification using two enzymes, reverse transcriptase and Taq polymerase. A modified method was developed to improve RT-PCR assays. The improved method was as sensitive in NV and HAV detection the traditional two-enzyme method. These improvements to viral nucleic acid detection have the potential to improve sensitivity, specificity and confidence in RT-PCR results.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalWater Science and Technology
Volume38
Issue number12
DOIs
StatePublished - Dec 1 1998
Externally publishedYes
EventProceedings of the 1998 19th Biennial Conference of the International Association on Water Quality,. Part 10 (of 10) - Vancouver, Can
Duration: Jun 21 1998Jun 26 1998

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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