Improvements in the RT-PCR detection of enteric viruses in environmental samples

K. J. Schwab, F. H. Neill, M. K. Estes, R. L. Atmar

Research output: Contribution to journalConference article

Abstract

The current methods for the detection of nucleic acid from enteric viruses in environmental samples usually involve extensive concentration and purification of target viruses followed by RT-PCR amplification using two enzymes, reverse transcriptase and Taq polymerase. A modified method was developed to improve RT-PCR assays. The improved method was as sensitive in NV and HAV detection the traditional two-enzyme method. These improvements to viral nucleic acid detection have the potential to improve sensitivity, specificity and confidence in RT-PCR results.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalWater Science and Technology
Volume38
Issue number12
DOIs
StatePublished - Dec 1 1998
Externally publishedYes
EventProceedings of the 1998 19th Biennial Conference of the International Association on Water Quality,. Part 10 (of 10) - Vancouver, Can
Duration: Jun 21 1998Jun 26 1998

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

Fingerprint Dive into the research topics of 'Improvements in the RT-PCR detection of enteric viruses in environmental samples'. Together they form a unique fingerprint.

  • Cite this