Improvement of cellularity on cell block preparations using the so-called tissue coagulum clot method during endobronchial ultrasound-guided transbronchial fine-needle aspiration

Rex Chin Wei Yung, Susan Otell, Peter B Illei, Douglas P. Clark, David Feller-Kopman, Lonny Yarmus, Frederic B Askin, Edward Gabrielson, Qing Kay Li

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Cell block (CB) preparation during the endobronchial ultrasound-guided transbronchial fine-needle aspiration (EBUS-TBNA) procedure plays an important role in the diagnosis of lung cancer and recovery of cellular material for molecular characterization of the tumor. However, the efficiency of the conventional method of CB preparation is suboptimal. METHODS: In the current study, the "tissue coagulum clot" cell block (TCC-CB) method was used to prepare the CBs and its efficiency was compared with that of the conventional saline rinse cell block (NR-CB) method. A total of 84 consecutive TCC-CBs (106 lymph nodes [LNs] and 14 lung lesions) and 28 consecutive cases of NR-CB (39 LNs and 3 lung lesions) obtained within the same time period were included in the current study. RESULTS: In the TCC-CB specimens, 94 of 106 LN cases (88.7%) yielded sufficient diagnostic material, as did 11 of 14 lung lesions (78.6%). In the NRCB group, which was used as the control, 22 of 39 LN specimens (56.4%) and none of 3 lung specimens (0%) were found to provide sufficient diagnostic material. Although the average size of the LNs in the study group were not significantly different from those in the control group (1.76 cm vs 1.82 cm; P>.05), the overall nondiagnostic rates in the TCC-CB and NR-CB groups were 11.2% and 43.6%, respectively (P

Original languageEnglish (US)
Pages (from-to)185-195
Number of pages11
JournalCancer cytopathology
Volume120
Issue number3
DOIs
StatePublished - Jun 25 2012

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Fine Needle Biopsy
Lymph Nodes
Lung
Lung Neoplasms
Control Groups

Keywords

  • Cell block preparation
  • Endobronchial ultrasound-guided transbronchial fine-needle aspiration (EBUS-TBNA)
  • Fine-needle aspiration cytology
  • Improvement of cellularity
  • Lung cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

@article{4484ab6c311046fa82ec3f2e786308fb,
title = "Improvement of cellularity on cell block preparations using the so-called tissue coagulum clot method during endobronchial ultrasound-guided transbronchial fine-needle aspiration",
abstract = "BACKGROUND: Cell block (CB) preparation during the endobronchial ultrasound-guided transbronchial fine-needle aspiration (EBUS-TBNA) procedure plays an important role in the diagnosis of lung cancer and recovery of cellular material for molecular characterization of the tumor. However, the efficiency of the conventional method of CB preparation is suboptimal. METHODS: In the current study, the {"}tissue coagulum clot{"} cell block (TCC-CB) method was used to prepare the CBs and its efficiency was compared with that of the conventional saline rinse cell block (NR-CB) method. A total of 84 consecutive TCC-CBs (106 lymph nodes [LNs] and 14 lung lesions) and 28 consecutive cases of NR-CB (39 LNs and 3 lung lesions) obtained within the same time period were included in the current study. RESULTS: In the TCC-CB specimens, 94 of 106 LN cases (88.7{\%}) yielded sufficient diagnostic material, as did 11 of 14 lung lesions (78.6{\%}). In the NRCB group, which was used as the control, 22 of 39 LN specimens (56.4{\%}) and none of 3 lung specimens (0{\%}) were found to provide sufficient diagnostic material. Although the average size of the LNs in the study group were not significantly different from those in the control group (1.76 cm vs 1.82 cm; P>.05), the overall nondiagnostic rates in the TCC-CB and NR-CB groups were 11.2{\%} and 43.6{\%}, respectively (P",
keywords = "Cell block preparation, Endobronchial ultrasound-guided transbronchial fine-needle aspiration (EBUS-TBNA), Fine-needle aspiration cytology, Improvement of cellularity, Lung cancer",
author = "Yung, {Rex Chin Wei} and Susan Otell and Illei, {Peter B} and Clark, {Douglas P.} and David Feller-Kopman and Lonny Yarmus and Askin, {Frederic B} and Edward Gabrielson and Li, {Qing Kay}",
year = "2012",
month = "6",
day = "25",
doi = "10.1002/cncy.20199",
language = "English (US)",
volume = "120",
pages = "185--195",
journal = "Cancer cytopathology",
issn = "1934-662X",
publisher = "Wiley-Blackwell",
number = "3",

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TY - JOUR

T1 - Improvement of cellularity on cell block preparations using the so-called tissue coagulum clot method during endobronchial ultrasound-guided transbronchial fine-needle aspiration

AU - Yung, Rex Chin Wei

AU - Otell, Susan

AU - Illei, Peter B

AU - Clark, Douglas P.

AU - Feller-Kopman, David

AU - Yarmus, Lonny

AU - Askin, Frederic B

AU - Gabrielson, Edward

AU - Li, Qing Kay

PY - 2012/6/25

Y1 - 2012/6/25

N2 - BACKGROUND: Cell block (CB) preparation during the endobronchial ultrasound-guided transbronchial fine-needle aspiration (EBUS-TBNA) procedure plays an important role in the diagnosis of lung cancer and recovery of cellular material for molecular characterization of the tumor. However, the efficiency of the conventional method of CB preparation is suboptimal. METHODS: In the current study, the "tissue coagulum clot" cell block (TCC-CB) method was used to prepare the CBs and its efficiency was compared with that of the conventional saline rinse cell block (NR-CB) method. A total of 84 consecutive TCC-CBs (106 lymph nodes [LNs] and 14 lung lesions) and 28 consecutive cases of NR-CB (39 LNs and 3 lung lesions) obtained within the same time period were included in the current study. RESULTS: In the TCC-CB specimens, 94 of 106 LN cases (88.7%) yielded sufficient diagnostic material, as did 11 of 14 lung lesions (78.6%). In the NRCB group, which was used as the control, 22 of 39 LN specimens (56.4%) and none of 3 lung specimens (0%) were found to provide sufficient diagnostic material. Although the average size of the LNs in the study group were not significantly different from those in the control group (1.76 cm vs 1.82 cm; P>.05), the overall nondiagnostic rates in the TCC-CB and NR-CB groups were 11.2% and 43.6%, respectively (P

AB - BACKGROUND: Cell block (CB) preparation during the endobronchial ultrasound-guided transbronchial fine-needle aspiration (EBUS-TBNA) procedure plays an important role in the diagnosis of lung cancer and recovery of cellular material for molecular characterization of the tumor. However, the efficiency of the conventional method of CB preparation is suboptimal. METHODS: In the current study, the "tissue coagulum clot" cell block (TCC-CB) method was used to prepare the CBs and its efficiency was compared with that of the conventional saline rinse cell block (NR-CB) method. A total of 84 consecutive TCC-CBs (106 lymph nodes [LNs] and 14 lung lesions) and 28 consecutive cases of NR-CB (39 LNs and 3 lung lesions) obtained within the same time period were included in the current study. RESULTS: In the TCC-CB specimens, 94 of 106 LN cases (88.7%) yielded sufficient diagnostic material, as did 11 of 14 lung lesions (78.6%). In the NRCB group, which was used as the control, 22 of 39 LN specimens (56.4%) and none of 3 lung specimens (0%) were found to provide sufficient diagnostic material. Although the average size of the LNs in the study group were not significantly different from those in the control group (1.76 cm vs 1.82 cm; P>.05), the overall nondiagnostic rates in the TCC-CB and NR-CB groups were 11.2% and 43.6%, respectively (P

KW - Cell block preparation

KW - Endobronchial ultrasound-guided transbronchial fine-needle aspiration (EBUS-TBNA)

KW - Fine-needle aspiration cytology

KW - Improvement of cellularity

KW - Lung cancer

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U2 - 10.1002/cncy.20199

DO - 10.1002/cncy.20199

M3 - Article

C2 - 22144401

AN - SCOPUS:84864744400

VL - 120

SP - 185

EP - 195

JO - Cancer cytopathology

JF - Cancer cytopathology

SN - 1934-662X

IS - 3

ER -