Improved survival in free skin flap transfers in rats

Paul Manson, Kailash K. Narayan, Michael J. Im, Gregory B. Bulkley, John E. Hoopes

Research output: Contribution to journalArticle

Abstract

We have demonstrated previously that oxygen-derived free radicals are important mediators of tissue injury in experimental island skin flaps that have been subjected to prolonged ischemia (vascular occlusion) followed by reperfusion. In this study the role of oxygen free radicals in ischemia/reperfusion injury has been investigated in free flap transfers. Groin skin flaps were harvested, stored at room temperature for 21 to 24 hours, and transplanted to the contralateral groin. These free flap transfers normally exhibit a high incidence of complete necrosis. Treatment before the onset of reperfusion with a single dose of superoxide dismutase (SOD), a scavenger of Superoxide radicals, increased the survival rate of these skin flaps from 38% in the control group to 76% (p <0.025). Tissue levels of SOD were measured before ischemia, after ischemia but before reperfusion, and 30 minutes after reperfusion: untreated flap tissues, which were destined to undergo necrosis, exhibited a significant decrease in SOD activity after reperfusion, whereas SOD-treated flap tissues, destined to survive, demonstrated increased enzyme activity. High levels of tissue SOD activity thus appeared to be associated with improved flap survival. The results have significant clinical implications with regard to organ preservation and transplantation.

Original languageEnglish (US)
Pages (from-to)211-215
Number of pages5
JournalSurgery
Volume99
Issue number2
StatePublished - 1986

Fingerprint

Free Tissue Flaps
Superoxide Dismutase
Reperfusion
Skin
Ischemia
Groin
Free Radicals
Necrosis
Organ Preservation
Surgical Flaps
Organ Transplantation
Reperfusion Injury
Superoxides
Blood Vessels
Reactive Oxygen Species
Oxygen
Control Groups
Temperature
Incidence
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

Manson, P., Narayan, K. K., Im, M. J., Bulkley, G. B., & Hoopes, J. E. (1986). Improved survival in free skin flap transfers in rats. Surgery, 99(2), 211-215.

Improved survival in free skin flap transfers in rats. / Manson, Paul; Narayan, Kailash K.; Im, Michael J.; Bulkley, Gregory B.; Hoopes, John E.

In: Surgery, Vol. 99, No. 2, 1986, p. 211-215.

Research output: Contribution to journalArticle

Manson, P, Narayan, KK, Im, MJ, Bulkley, GB & Hoopes, JE 1986, 'Improved survival in free skin flap transfers in rats', Surgery, vol. 99, no. 2, pp. 211-215.
Manson P, Narayan KK, Im MJ, Bulkley GB, Hoopes JE. Improved survival in free skin flap transfers in rats. Surgery. 1986;99(2):211-215.
Manson, Paul ; Narayan, Kailash K. ; Im, Michael J. ; Bulkley, Gregory B. ; Hoopes, John E. / Improved survival in free skin flap transfers in rats. In: Surgery. 1986 ; Vol. 99, No. 2. pp. 211-215.
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