Improved surveillance of Japanese encephalitis by detection of virus-specific IgM in desiccated blood specimens

D. S. Burke, K. Chatiyanonda, S. Anandrik, S. Nakornsri, A. Nisalak, C. H. Hoke

Research output: Contribution to journalArticle

Abstract

An IgM antibody-capture type enzyme-linked immunoassay (MAC ELISA) was compared with the haemagglutination inhibition method (HI) for establishing a laboratory diagnosis of acute Japanese encephalitis (JE) virus infection using specimens of dried blood eluted from filter paper strips. Paired samples from 243 encephalitis patients, which had been obtained by mail through a national surveillance programme in Thailand, were tested. During the peak of the 1983 encephalitis epidemic, 72% of cases were diagnosed as Japanese encephalitis by MAC ELISA, compared with only 38% by HI. During nonepidemic periods, the proportions diagnosed as Japanese encephalitis by MAC ELISA or HI were 26% and 33%, respectively. Detection of IgM anti-JE activity by the antibody-capture immunoassay is superior to the HI method for establishing a diagnosis of acute Japanese encephalitis using dried blood specimens.

Original languageEnglish (US)
Pages (from-to)1037-1042
Number of pages6
JournalBulletin of the World Health Organization
Volume63
Issue number6
StatePublished - 1985
Externally publishedYes

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Japanese Encephalitis Virus
Japanese Encephalitis
Immunoglobulin M
Hemagglutination
Enzyme-Linked Immunosorbent Assay
Arbovirus Encephalitis
Antibodies
Clinical Laboratory Techniques
Postal Service
Encephalitis
Thailand
Immunoenzyme Techniques
Immunoassay
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Burke, D. S., Chatiyanonda, K., Anandrik, S., Nakornsri, S., Nisalak, A., & Hoke, C. H. (1985). Improved surveillance of Japanese encephalitis by detection of virus-specific IgM in desiccated blood specimens. Bulletin of the World Health Organization, 63(6), 1037-1042.

Improved surveillance of Japanese encephalitis by detection of virus-specific IgM in desiccated blood specimens. / Burke, D. S.; Chatiyanonda, K.; Anandrik, S.; Nakornsri, S.; Nisalak, A.; Hoke, C. H.

In: Bulletin of the World Health Organization, Vol. 63, No. 6, 1985, p. 1037-1042.

Research output: Contribution to journalArticle

Burke, DS, Chatiyanonda, K, Anandrik, S, Nakornsri, S, Nisalak, A & Hoke, CH 1985, 'Improved surveillance of Japanese encephalitis by detection of virus-specific IgM in desiccated blood specimens', Bulletin of the World Health Organization, vol. 63, no. 6, pp. 1037-1042.
Burke, D. S. ; Chatiyanonda, K. ; Anandrik, S. ; Nakornsri, S. ; Nisalak, A. ; Hoke, C. H. / Improved surveillance of Japanese encephalitis by detection of virus-specific IgM in desiccated blood specimens. In: Bulletin of the World Health Organization. 1985 ; Vol. 63, No. 6. pp. 1037-1042.
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