Improved static lung preservation with corticosteroids and hypothermia

T. S. Hall, A. M. Borkon, G. C. Gurtner, J. Brawn, G. M. Hutchins, B. A. Reitz, William A Baumgartner

Research output: Contribution to journalArticle

Abstract

With methylprednisolone as a chemical inhibitor of leukocytes, extended preservation was conducted with an isolated rabbit lung model. The heart-lung blocks of 39 New Zealand white rabbits were flushed in situ with 100 ml of Euro-Collins' solution, harvested, inflated (70%), and preserved at 4°C. Lungs immediately reperfused with whole blood (control lungs, group 1) were compared with lung preserved without methylprednisolone for 5, 12, and 24 hours (groups 2 to 4) and those preserved with methylprednisolone for 12 and 24 hours (groups 5 and 6, respectively). Methylprednisolone (30 mg/kg) was administered before harvest and was used as an additive to the flush and in the blood reperfusate. Hypothermia and Euro-Collins' flush alone provided adequate preservation for up to 5 hours; however, lung edema was evident by 12 hours of cold ischemia and became severe by 24 hours. By all measured parameters, the lung in group 5 (treated with methylprednisolone) demonstrated values equal to or better than control lungs. By 24 hours of preservation the beneficial effects of the steroid treatment were no longer evident. Histologic evaluation revealed mild to moderate injury after 5 hours of cold ischemia; progressive edema and hemorrhage were found after 12 and 24 hours of preservation. This injury was significantly ameliorated by methylprednisolone treatment at 12 hours but not at 24 hours. This study suggests that static preservation for up to 5 hours is possible with hypothermia and a Euro-Collins' flush and that extended preservation to 12 hours is possible with pharmacologic dosages of methylprednisolone.

Original languageEnglish (US)
Pages (from-to)348-352
Number of pages5
JournalJournal of Heart Transplantation
Volume7
Issue number5
StatePublished - 1988

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Hypothermia
Methylprednisolone
Adrenal Cortex Hormones
Lung
Cold Ischemia
Edema
Rabbits
Heart Block
Wounds and Injuries
Leukocytes
Steroids
Hemorrhage
Control Groups
Therapeutics

ASJC Scopus subject areas

  • Transplantation

Cite this

Hall, T. S., Borkon, A. M., Gurtner, G. C., Brawn, J., Hutchins, G. M., Reitz, B. A., & Baumgartner, W. A. (1988). Improved static lung preservation with corticosteroids and hypothermia. Journal of Heart Transplantation, 7(5), 348-352.

Improved static lung preservation with corticosteroids and hypothermia. / Hall, T. S.; Borkon, A. M.; Gurtner, G. C.; Brawn, J.; Hutchins, G. M.; Reitz, B. A.; Baumgartner, William A.

In: Journal of Heart Transplantation, Vol. 7, No. 5, 1988, p. 348-352.

Research output: Contribution to journalArticle

Hall, TS, Borkon, AM, Gurtner, GC, Brawn, J, Hutchins, GM, Reitz, BA & Baumgartner, WA 1988, 'Improved static lung preservation with corticosteroids and hypothermia', Journal of Heart Transplantation, vol. 7, no. 5, pp. 348-352.
Hall TS, Borkon AM, Gurtner GC, Brawn J, Hutchins GM, Reitz BA et al. Improved static lung preservation with corticosteroids and hypothermia. Journal of Heart Transplantation. 1988;7(5):348-352.
Hall, T. S. ; Borkon, A. M. ; Gurtner, G. C. ; Brawn, J. ; Hutchins, G. M. ; Reitz, B. A. ; Baumgartner, William A. / Improved static lung preservation with corticosteroids and hypothermia. In: Journal of Heart Transplantation. 1988 ; Vol. 7, No. 5. pp. 348-352.
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