Improved selective ion monitoring mass-spectrometric assay for the determination of n,n-dimethyltryptamine in human blood utilizing capillary column gas chromatography

R. W. Walker, L. R. Mandel, Joel Kleinman, J. C. Gillin, R. J. Wyatt, W. J A Vandenheuvel

Research output: Contribution to journalArticle

Abstract

A gas-liquid chromatographic-mass spectrometric procedure is described for the assay of dimethyltryptamine (DMT) in whole blood. The use of a glass capillary column in combination with selective ion monitoring results in an assay with a high degree of specificity and sensitivity. 5-Methoxy-DMT is used as an internal standard and carrier in the isolation procedure. The superior resolving characteristics of the capillary column (as compared to previously employed packed columns) allows monitoring of the intense m/e 58 ion arising from the DMT side-chain. A sensitivity limit of 10 pg/ml blood is realized with a 10-ml blood sample.

Original languageEnglish (US)
Pages (from-to)539-546
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume162
Issue number4
DOIs
StatePublished - Apr 11 1979
Externally publishedYes

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N,N-Dimethyltryptamine
Column chromatography
Gas chromatography
Gas Chromatography
Assays
Blood
Ions
Monitoring
Glass
Gases
Sensitivity and Specificity
Liquids

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Improved selective ion monitoring mass-spectrometric assay for the determination of n,n-dimethyltryptamine in human blood utilizing capillary column gas chromatography. / Walker, R. W.; Mandel, L. R.; Kleinman, Joel; Gillin, J. C.; Wyatt, R. J.; Vandenheuvel, W. J A.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 162, No. 4, 11.04.1979, p. 539-546.

Research output: Contribution to journalArticle

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