Improved method for the determination of 4′-epidoxorubicin and seven metabolites in plasma by high-performance liquid chromatography

P. A. Maessen, K. B. Mross, H. M. Pinedo, W. J F Van Der Vijgh

Research output: Contribution to journalArticle

Abstract

4′-Epidoxorubicin, its seven metabolites and doxorubicin, as internal standard, were efficiently extracted from plasma using C18 Sep-Pak cartridges. The recoveries ranged from 58% for doxorubicin aglycone up to 98% for 4′-epidoxorubicin glucuronide. The anthracyclines were separated by reversed-phase high-performance liquid chromatography within 9 min and analysed by fluorescence. The assay was sensitive to 3·10-10 M for the glucuronides up to 12·10-10 M for 7-deoxydoxorubicin aglycone. The peak-height ratio of the fluorescence intensities of the anthracyclines versus doxorubicin showed a linear correlation with the concentration from the detection limit up to 2.5·10-7 M (correlation coefficient r2 > 0.99). Within-day and between-day precision of the assay were in the ranges 2-14% (n = 6) and 2-11% (n = 6), respectively.

Original languageEnglish (US)
Pages (from-to)339-346
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume417
Issue numberC
DOIs
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

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