TY - JOUR
T1 - Improved light microscopy counting method for accurately counting Plasmodium parasitemia and reticulocytemia
AU - Lim, Caeul
AU - Pereira, Ligia
AU - Shardul, Pritish
AU - Mascarenhas, Anjali
AU - Maki, Jennifer
AU - Rixon, Jordan
AU - Shaw-Saliba, Kathryn
AU - White, John
AU - Silveira, Maria
AU - Gomes, Edwin
AU - Chery, Laura
AU - Rathod, Pradipsinh K.
AU - Duraisingh, Manoj T.
N1 - Publisher Copyright:
© 2016 Wiley Periodicals, Inc.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Even with the advances in molecular or automated methods for detection of red blood cells of interest (such as reticulocytes or parasitized cells), light microscopy continues to be the gold standard especially in laboratories with limited resources. The conventional method for determination of parasitemia and reticulocytemia uses a Miller reticle, a grid with squares of different sizes. However, this method is prone to errors if not used correctly and counts become inaccurate and highly time-consuming at low frequencies of target cells. In this report, we outline the correct guidelines to follow when using a reticle for counting, and present a new counting protocol that is a modified version of the conventional method for increased accuracy in the counting of low parasitemias and reticulocytemias. Am. J. Hematol. 91:852–855, 2016.
AB - Even with the advances in molecular or automated methods for detection of red blood cells of interest (such as reticulocytes or parasitized cells), light microscopy continues to be the gold standard especially in laboratories with limited resources. The conventional method for determination of parasitemia and reticulocytemia uses a Miller reticle, a grid with squares of different sizes. However, this method is prone to errors if not used correctly and counts become inaccurate and highly time-consuming at low frequencies of target cells. In this report, we outline the correct guidelines to follow when using a reticle for counting, and present a new counting protocol that is a modified version of the conventional method for increased accuracy in the counting of low parasitemias and reticulocytemias. Am. J. Hematol. 91:852–855, 2016.
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U2 - 10.1002/ajh.24383
DO - 10.1002/ajh.24383
M3 - Article
C2 - 27074559
AN - SCOPUS:84978296444
SN - 0361-8609
VL - 91
SP - 852
EP - 855
JO - American journal of hematology
JF - American journal of hematology
IS - 8
ER -