Improved in vitro development of porcine embryos with different energy substrates and serum

Hye Soo Kim, Gab Sang Lee, Sang Hwan Hyun, So Hyun Lee, Dong Hyun Nam, Yeon Woo Jeong, Sue Kim, Sung Keun Kang, Byeong Chun Lee, Woo Suk Hwang

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of replacing 5.5mM glucose in North Carolina State University (NCSU)-23 medium with 0.5mM pyruvate/5.0mM lactate on porcine IVF embryo development was investigated in Experiment 1. Culturing embryos with pyruvate/lactate for 7 days or with pyruvate/lactate from Days 0 to 2, and then glucose from Days 2 to 7 improved cleavage rates. In Experiment 2, embryos were cultured for 7 days in pyruvate/lactate containing NCSU-23 medium supplemented with 0.05% PVA, 0.4% BSA or 10% fetal bovine serum (FBS). The BSA supplement increased the rates of cleavage, blastocyst formation, and the number of total cells in blastocysts. In Experiment 3, embryos were cultured in pyruvate/ lactate containing NCSU-23 medium supplemented with 0.4% BSA for 7 days (BSA-PL), 0.4% BSA from Days 0 to 4 and then 10% FBS from Days 4 to 7 (BSA-PL→F) or 0.4% BSA from Days 0 to 7 with addition of 10% FBS (BSA-PL+F) at Day 4. More blastocysts in BSA-PL→F and hatching or hatched blastocysts in BSA-PL→F and BSA-PL+F were obtained. Total cell number in blastocysts derived from BSA-PL→F and BSA-PL+F were increased. Our results demonstrated that supplementing pyruvate/lactate containing NCSU-23 medium with 0.4% BSA for 4 days and replacing it with 10% FBS for another 3 days improved porcine IVF embryo development.

Original languageEnglish (US)
Pages (from-to)1381-1393
Number of pages13
JournalTheriogenology
Volume61
Issue number7-8
DOIs
StatePublished - May 2004
Externally publishedYes

Keywords

  • Blastocyst
  • Cell number
  • Energy substrate
  • Macromolecule
  • Porcine embryos

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

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