Improved hydrophilic interaction chromatography method for the identification and quantification of glucosinolates

Kristina L. Wade, Ian J. Garrard, Jed W Fahey

Research output: Contribution to journalArticle

Abstract

An improved hydrophilic interaction liquid chromatography (HILIC) method has been developed to separate members of a closely related family of chemoprotective phytochemicals called glucosinolates. This method exploits the emergence of a second generation of HILIC chemistry, using a silica-based permanently zwitterionic stationary phase. These columns are more robust, durable, and glucosinolates separations are more reproducible than with the original polyhydroxyethyl aspartamide columns. Furthermore, the HILIC system that we report herein permits much greater alteration of the mobile phase composition for customized separation of glucosinolates from plant extracts, across a wide spectrum of polarity.

Original languageEnglish (US)
Pages (from-to)469-472
Number of pages4
JournalJournal of Chromatography A
Volume1154
Issue number1-2
DOIs
StatePublished - Jun 22 2007

Fingerprint

Glucosinolates
Liquid chromatography
Chromatography
Hydrophobic and Hydrophilic Interactions
Liquid Chromatography
Plant Extracts
Phytochemicals
Phase composition
Silicon Dioxide

Keywords

  • Broccoli
  • Chemoprotection
  • Glucoiberin
  • Glucoraphanin
  • HPLC
  • Phytochemical
  • Sulforaphane glucosinolate

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Improved hydrophilic interaction chromatography method for the identification and quantification of glucosinolates. / Wade, Kristina L.; Garrard, Ian J.; Fahey, Jed W.

In: Journal of Chromatography A, Vol. 1154, No. 1-2, 22.06.2007, p. 469-472.

Research output: Contribution to journalArticle

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