Improved DNA electrophoresis in conditions favoring polyborates and lewis acid complexation

Hari Singhal; Yunzhao R. Ren; Scott E. Kern

doi:10.1371/journal.pone.0011318

Improved DNA electrophoresis in conditions favoring polyborates and lewis acid complexation

Hari Singhal, Yunzhao R. Ren, Scott E. Kern

School of Medicine

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Spatial compression among the longer DNA fragments occurs during DNA electrophoresis in agarose and non-agarose gels when using certain ions in the conductive buffer, impairing the range of fragment sizes resolved well in a single gel. Substitutions using various polyhydroxyl anions supported the underlying phenomenon as the complexation of Lewis acids to DNA. We saw significant improvements using conditions (lithium borate 10 mM cations, pH 6.5) favoring the formation of borate polyanions and having lower conductance and Joule heating, delayed electrolyte exhaustion, faster electrophoretic run-speed, and sharper separation of DNA bands from 100bp to 12 kb in a single run.

Original language	English (US)
Article number	e11318
Journal	PloS one
Volume	5
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0011318
State	Published - 2010

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General

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Improved DNA electrophoresis in conditions favoring polyborates and lewis acid complexation

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