Improved DNA electrophoresis in conditions favoring polyborates and lewis acid complexation

Hari Singhal, Yunzhao R. Ren, Scott E. Kern

Research output: Contribution to journalArticlepeer-review

Abstract

Spatial compression among the longer DNA fragments occurs during DNA electrophoresis in agarose and non-agarose gels when using certain ions in the conductive buffer, impairing the range of fragment sizes resolved well in a single gel. Substitutions using various polyhydroxyl anions supported the underlying phenomenon as the complexation of Lewis acids to DNA. We saw significant improvements using conditions (lithium borate 10 mM cations, pH 6.5) favoring the formation of borate polyanions and having lower conductance and Joule heating, delayed electrolyte exhaustion, faster electrophoretic run-speed, and sharper separation of DNA bands from 100bp to 12 kb in a single run.

Original languageEnglish (US)
Article numbere11318
JournalPloS one
Volume5
Issue number6
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Improved DNA electrophoresis in conditions favoring polyborates and lewis acid complexation'. Together they form a unique fingerprint.

Cite this