Improved diagnostic medium for separation of Cryptococcus neoformans var. neoformans (Serotypes A and D) and Cryptococcus neoformans var. gattii (serotypes B and C)

K. J. Kwon-Chung, I. Polacheck, J. E. Bennett

Research output: Contribution to journalArticlepeer-review

Abstract

A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D (Cryptococcus neoformans var. neoformans) and serotype B or C (C. neoformans var. gattii) than creatinine-dextrose-bromthymol blue or glycine-cycloheximide-phenol red media. Among 143 isolates of serotype A or D and 70 isolates of serotype B or C, the new medium correlated completely with the serotype, whereas nearly 11% of these isolates gave discrepant reactions with creatinine-dextrose-bromthymol blue and glycine-cycloheximide-phenol red media.

Original languageEnglish (US)
Pages (from-to)535-537
Number of pages3
JournalJournal of clinical microbiology
Volume15
Issue number3
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Microbiology (medical)

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