Improved detection of human immunodeficiency virus type 1 variants by analysis of replicate amplification reactions: Relevance to studies of human immunodeficiency virus type 1 vertical transmission

Graziella Becker-Pergola, Jenny L. Mellquist, James R. Eshleman, J. Brooks Jackson, Susan H. Eshleman

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Human immunodeficiency virus type 1 (HIV-1)-infected individuals typically harbor mixtures of HIV-1 variants. For HIV-1 transmission studies, methods used for genotypic analysis should reliably detect variant mixtures. Such studies typically analyze complementary DNAs (cDNAs) from a single polymerase chain reaction (PCR) amplification. This approach may fail to detect variant mixtures in some samples because of analytic bias. Methods: To evaluate the impact of analytic bias on the detection of HIV-1 variants, we analyzed samples from a mother and infant known to contain both subtypes A and D HIV-1. The env third variable region of HIV-1 gp120 (V3 region) was amplified and cloned in five replicate experiments using a single plasma sample from each individual. Ten cDNAs from each experiment were analyzed. Results: The subtype mixture was detected in only four of 10 amplification experiments (three of five for the mother and one of five for the infant). Sequencing of uncloned PCR products showed that a single subtype, either A or D, was preferentially amplified in each experiment. However, the subtype mixture was detected for each sample by analyzing the five replicate experiments as a group. Conclusions: This shows that mixtures of HIV-1 variants may be more readily detected when replicate amplification reactions are analyzed. This approach may be useful for characterizing HIV-1 variants for studies of HIV-1 transmission.

Original languageEnglish (US)
Pages (from-to)261-268
Number of pages8
JournalMolecular Diagnosis
Volume4
Issue number4
DOIs
StatePublished - Jan 1 1999

Keywords

  • Clade
  • Dual infection
  • Perinatal
  • Subtype

ASJC Scopus subject areas

  • Medicine(all)

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