A new amplification technique is described for the detection of small amounts of antigen in ethanol‐fixed cytologic specimens and formalin‐fixed tissues. By adding a third antibody layer to the already sensitive avidin‐biotin immunoperoxidase system. we have demonstrated a considerable increase in both the quantitative and qualitative aspects of this immunostaining method. In evaluating this technique, we employed a previously defined small cell lung carcinoma cell surface antigen system and monoclonal antibodies.
- Avidin‐biotin complex method
- Monoclonal antibodies
ASJC Scopus subject areas
- Pathology and Forensic Medicine