Improved antigen detection in ethanol‐fixed cytologic specimens. A modified avidin‐biotin‐peroxidase complex (ABC) method

Prabodh K. Gupta, Joseph D. Myers, Stephen B. Baylin, James L. Mulshine, Frank Cuttitta, Adi F. Gazdar

Research output: Contribution to journalArticlepeer-review

Abstract

A new amplification technique is described for the detection of small amounts of antigen in ethanol‐fixed cytologic specimens and formalin‐fixed tissues. By adding a third antibody layer to the already sensitive avidin‐biotin immunoperoxidase system. we have demonstrated a considerable increase in both the quantitative and qualitative aspects of this immunostaining method. In evaluating this technique, we employed a previously defined small cell lung carcinoma cell surface antigen system and monoclonal antibodies.

Original languageEnglish (US)
Pages (from-to)133-136
Number of pages4
JournalDiagnostic cytopathology
Volume1
Issue number2
DOIs
StatePublished - 1985

Keywords

  • Avidin‐biotin complex method
  • Immunocytochemistry
  • Immunoperoxidase
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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