Improved and expanded Q-system reagents for genetic manipulations

Olena Riabinina, David Luginbuhl, Elizabeth Marr, Sha Liu, Mark N. Wu, Liqun Luo, Christopher J. Potter

Research output: Contribution to journalArticle

Abstract

The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.

Original languageEnglish (US)
Pages (from-to)219-222
Number of pages4
JournalNature Methods
Volume12
Issue number3
DOIs
StatePublished - Feb 26 2015

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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