Importance of prostanoids in calcium chelator-induced airway constriction

Karen Sue Lindeman, N. Downey, Mark Chang Hwa Liu, W. C. Hubbard, A. Kagey-Sobotka, C. A. Hirshman

Research output: Contribution to journalArticle

Abstract

To elucidate the mechanism of calcium chelator-induced airway constriction, we examined the relationship between prostanoids and histamine in bronchoalveolar lavage (BAL) fluid and the magnitude of airway constriction in peripheral airways of anesthetized Basenji-Greyhound dogs. A wedged bronchoscope technique was used to measure collateral system resistance (Rcs) before and after aerosol challenges. Sublobar segments were challenged either with acetylcholine or with Na2EDTA in the presence or absence of meclofenamate sodium (3 mg/kg iv) or methylprednisolone (2 mg · kg-1 · day-1). After measurements of Rcs, BAL was performed, and the fluid was analyzed for prostanoids with the use of gas chromatography-mass spectrometry. Sublobar segments challenged with Na2EDTA showed increased concentrations of prostaglandin (PG) D2 but no increases in PGE2, PGF(2α), 9α,11β-PGF2, 6-keto-PGF(1α), thromboxane B2, or histamine. There was a strong relationship (r = 0.84, P = 0.005) between changes in Rcs after Na2EDTA and concentrations of PGD2 in BAL fluid. Acetylcholine, which increased Rcs to a similar degree as Na2EDTA did, produced no significant increase in prostanoid and concentrations. Changes in Rcs after Na2EDTA and concentrations of PGD2 were reduced in the presence of meclofenamate or methylprednisolone. These data support the idea that the mechanism of calcium chelator-induced bronchoconstriction involves the release of bronchoconstricting prostanoids.

Original languageEnglish (US)
Pages (from-to)1110-1116
Number of pages7
JournalJournal of Applied Physiology
Volume75
Issue number3
StatePublished - 1993

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Keywords

  • bronchoalveolar lavage
  • bronchoconstriction
  • collateral resistance
  • ethylenediaminetetraacetic acid
  • histamine

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Lindeman, K. S., Downey, N., Liu, M. C. H., Hubbard, W. C., Kagey-Sobotka, A., & Hirshman, C. A. (1993). Importance of prostanoids in calcium chelator-induced airway constriction. Journal of Applied Physiology, 75(3), 1110-1116.