Importance of mitochondria in survival of Cryptococcus neoformans under low oxygen conditions and tolerance to cobalt chloride

Susham S. Ingavale, Yun C. Chang, Hyeseung Lee, Carol M. McClelland, Madeline L. Leong, Kyung J. Kwon-Chung

Research output: Contribution to journalArticle

Abstract

Cryptococcus neoformans is an environmental fungal pathogen that requires atmospheric levels of oxygen for optimal growth. For the fungus to be able to establish an infection, it must adapt to the low oxygen concentrations in the host environment compared to its natural habitat. In order to investigate the oxygen sensing mechanism in C. neoformans, we screened T-DNA insertional mutants for hypoxia-mimetic cobalt chloride (CoCl2)-sensitive mutants. All the CoCl2-sensitive mutants had a growth defect under low oxygen conditions at 37°C. The majority of mutants are compromised in their mitochondrial function, which is reflected by their reduced rate of respiration. Some of the mutants are also defective in mitochondrial membrane permeability, suggesting the importance of an intact respiratory system for survival under both high concentrations of CoCl2 as well as low oxygen conditions. In addition, the mutants tend to accumulate intracellular reactive oxygen species (ROS), and all mutants show sensitivity to various ROS generating chemicals. Gene expression analysis revealed the involvement of several pathways in response to cobalt chloride. Our findings indicate cobalt chloride sensitivity and/or sensitivity to low oxygen conditions are linked to mitochondrial function, sterol and iron homeostasis, ubiquitination, and the ability of cells to respond to ROS. These findings imply that multiple pathways are involved in oxygen sensing in C. neoformans.

Original languageEnglish (US)
Article numbere1000155
JournalPLoS Pathogens
Volume4
Issue number9
DOIs
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

Cryptococcus neoformans
Mitochondria
Oxygen
Reactive Oxygen Species
Ubiquitination
Mitochondrial Membranes
Sterols
Respiratory Rate
Growth
cobaltous chloride
Respiratory System
Ecosystem
Permeability
Homeostasis
Fungi
Iron
Gene Expression
Infection

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Ingavale, S. S., Chang, Y. C., Lee, H., McClelland, C. M., Leong, M. L., & Kwon-Chung, K. J. (2008). Importance of mitochondria in survival of Cryptococcus neoformans under low oxygen conditions and tolerance to cobalt chloride. PLoS Pathogens, 4(9), [e1000155]. https://doi.org/10.1371/journal.ppat.1000155

Importance of mitochondria in survival of Cryptococcus neoformans under low oxygen conditions and tolerance to cobalt chloride. / Ingavale, Susham S.; Chang, Yun C.; Lee, Hyeseung; McClelland, Carol M.; Leong, Madeline L.; Kwon-Chung, Kyung J.

In: PLoS Pathogens, Vol. 4, No. 9, e1000155, 09.2008.

Research output: Contribution to journalArticle

Ingavale, Susham S. ; Chang, Yun C. ; Lee, Hyeseung ; McClelland, Carol M. ; Leong, Madeline L. ; Kwon-Chung, Kyung J. / Importance of mitochondria in survival of Cryptococcus neoformans under low oxygen conditions and tolerance to cobalt chloride. In: PLoS Pathogens. 2008 ; Vol. 4, No. 9.
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