Import of proteins into yeast mitochondria: the nuclear MAS2 gene encodes a component of the processing protease that is homologous to the MAS1-encoded subunit.

R. E. Jensen, M. P. Yaffe

Research output: Contribution to journalArticle

Abstract

The mas2 mutant of Saccharomyces cerevisiae is temperature sensitive for import of proteins into mitochondria. To identify the lesion in this mutant, we have cloned and sequenced the wild-type MAS2 gene and determined the intracellular location of its protein product. MAS2 encodes an essential 53-kd protein that is located in the mitochondrial matrix and is homologous to the MAS1 protein, a previously identified subunit of the protease that cleaves presequences from mitochondrial precursor proteins. The activity of this enzyme is temperature sensitive in mas2 cells. Together with the results of the accompanying study these results show that MAS2 and MAS1 encode the two subunits of the processing protease.

Original languageEnglish (US)
Pages (from-to)3863-3871
Number of pages9
JournalThe EMBO journal
Volume7
Issue number12
StatePublished - Dec 1 1988
Externally publishedYes

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Mitochondria
Fungal Proteins
Peptide Hydrolases
Genes
Processing
Proteins
Temperature
Protein Precursors
Mitochondrial Proteins
Yeast
Saccharomyces cerevisiae
Enzymes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Import of proteins into yeast mitochondria : the nuclear MAS2 gene encodes a component of the processing protease that is homologous to the MAS1-encoded subunit. / Jensen, R. E.; Yaffe, M. P.

In: The EMBO journal, Vol. 7, No. 12, 01.12.1988, p. 3863-3871.

Research output: Contribution to journalArticle

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