Implications of using ED-50 and probit analysis in comparing retinal injury threshold data

D. H. Sliney; J. Mellerio; K. Schulmeister

doi:10.1117/12.426711

Implications of using ED-50 and probit analysis in comparing retinal injury threshold data

D. H. Sliney, J. Mellerio, K. Schulmeister

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

An indication of the level of uncertainty in laser injury studies relates to the slope of the transformed dose-response curve, or the "probit plot" of the data. The most cited threshold in a laser injury experiment is the point on the probit plot that represents a 50 % probability of injury: the ED-50. This value is frequently referred to as the "threshold", even though some experimental damage points exist below this "threshold." An analysis of any number of example data sets reveals that the slope in most experiments could not be explained by biological variation alone. The optical, thermophysical and biological factors influencing the probit plot are critically analyzed. By theoretically modelling an experiment, small errors in focus are shown to produce a substantial change in the ED-50 and the slope of the probit plot. The implications of plotting spot-size dependence with ED-50 values are shown to be significant, and can lead to erroneous conclusions regarding the apparent spot-size dependence.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	B.E. Stuck, M. Belkin
Pages	128-137
Number of pages	10
Volume	4246
DOIs	https://doi.org/10.1117/12.426711
State	Published - 2001
Externally published	Yes
Event	Laser and Noncoherent Light Ocular Effects:Epidemiology, Prevention, and Treatment - San Jose, CA, United States Duration: Jan 22 2001 → Jan 22 2001

Other

Other	Laser and Noncoherent Light Ocular Effects:Epidemiology, Prevention, and Treatment
Country/Territory	United States
City	San Jose, CA
Period	1/22/01 → 1/22/01

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

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10.1117/12.426711

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Sliney, DH, Mellerio, J & Schulmeister, K 2001, Implications of using ED-50 and probit analysis in comparing retinal injury threshold data. in BE Stuck & M Belkin (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4246, pp. 128-137, Laser and Noncoherent Light Ocular Effects:Epidemiology, Prevention, and Treatment, San Jose, CA, United States, 1/22/01. https://doi.org/10.1117/12.426711

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title = "Implications of using ED-50 and probit analysis in comparing retinal injury threshold data",

abstract = "An indication of the level of uncertainty in laser injury studies relates to the slope of the transformed dose-response curve, or the {"}probit plot{"} of the data. The most cited threshold in a laser injury experiment is the point on the probit plot that represents a 50 % probability of injury: the ED-50. This value is frequently referred to as the {"}threshold{"}, even though some experimental damage points exist below this {"}threshold.{"} An analysis of any number of example data sets reveals that the slope in most experiments could not be explained by biological variation alone. The optical, thermophysical and biological factors influencing the probit plot are critically analyzed. By theoretically modelling an experiment, small errors in focus are shown to produce a substantial change in the ED-50 and the slope of the probit plot. The implications of plotting spot-size dependence with ED-50 values are shown to be significant, and can lead to erroneous conclusions regarding the apparent spot-size dependence.",

author = "Sliney, {D. H.} and J. Mellerio and K. Schulmeister",

year = "2001",

doi = "10.1117/12.426711",

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N2 - An indication of the level of uncertainty in laser injury studies relates to the slope of the transformed dose-response curve, or the "probit plot" of the data. The most cited threshold in a laser injury experiment is the point on the probit plot that represents a 50 % probability of injury: the ED-50. This value is frequently referred to as the "threshold", even though some experimental damage points exist below this "threshold." An analysis of any number of example data sets reveals that the slope in most experiments could not be explained by biological variation alone. The optical, thermophysical and biological factors influencing the probit plot are critically analyzed. By theoretically modelling an experiment, small errors in focus are shown to produce a substantial change in the ED-50 and the slope of the probit plot. The implications of plotting spot-size dependence with ED-50 values are shown to be significant, and can lead to erroneous conclusions regarding the apparent spot-size dependence.

AB - An indication of the level of uncertainty in laser injury studies relates to the slope of the transformed dose-response curve, or the "probit plot" of the data. The most cited threshold in a laser injury experiment is the point on the probit plot that represents a 50 % probability of injury: the ED-50. This value is frequently referred to as the "threshold", even though some experimental damage points exist below this "threshold." An analysis of any number of example data sets reveals that the slope in most experiments could not be explained by biological variation alone. The optical, thermophysical and biological factors influencing the probit plot are critically analyzed. By theoretically modelling an experiment, small errors in focus are shown to produce a substantial change in the ED-50 and the slope of the probit plot. The implications of plotting spot-size dependence with ED-50 values are shown to be significant, and can lead to erroneous conclusions regarding the apparent spot-size dependence.

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ER -

Implications of using ED-50 and probit analysis in comparing retinal injury threshold data

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