TY - JOUR
T1 - Implication of the hypoxia response element of the Vegf promoter in mouse models of retinal and choroidal neovascularization, but not retinal vascular development
AU - Vinores, Stanley A.
AU - Xiao, Wei Hong
AU - Aslam, Sadia
AU - Shen, Jikui
AU - Oshima, Yuji
AU - Nambu, Hiroyuki
AU - Liu, Hansheng
AU - Carmeliet, Peter
AU - Campochiaro, Peter A.
PY - 2006/3
Y1 - 2006/3
N2 - Retinal neovascularization (NV) and macular edema, resulting from blood-retinal barrier (BRB) breakdown, are major causes of visual loss in ischemic retinopathies. Choroidal NV (CNV) occurs in diseases of the retinal pigmented epithelium/Bruch's membrane complex and is another extremely prevalent cause of visual loss. We used mice in which the hypoxia response element (HRE) is deleted from the vascular endothelialg rowth factor(vegf) promoter (Vegf δ/δ mice) to explore the role of induction of VEGF through the HRE in these disease processes. Compared to wild type (Vegf δ/δ) mice with oxygen-induced ischemic retinopathy (OIR) in which vegf mRNA levels were increased and prominent retinal NV and BRB breakdown occurred, Vegfδ/δ littermates with OIR failed to increase vegf mRNA levels in the retina and had significantly less retinal NV and BRB breakdown, but showed prominent dilation of some superficial retinal vessels. Vegf+/δ littermates with ischemic retinopathy developed comparable retinal NV to Vegf+/+ mice, exhibited intermediate levels of BRB breakdown, and did not show vasodilation. In a mouse model of CNV, due to laser-induced rupture of Bruch's membrane, the area of CNV at Bruch's membrane rupture sites was more than tenfold greater in Vegf +/+ mice than in Vegfδ/δ littermates. In contrast to these dramatic differences in pathologic ocular NV, Vegf δ/δ mice showed subtle differences in retinal vascular development compared to Vegf+/+ mice; it was slightly delayed, but otherwise normal. These data suggest that induction of VEGF through the HRE in its promoter is critical for retinal and CNV, but not for retinal vascular development.
AB - Retinal neovascularization (NV) and macular edema, resulting from blood-retinal barrier (BRB) breakdown, are major causes of visual loss in ischemic retinopathies. Choroidal NV (CNV) occurs in diseases of the retinal pigmented epithelium/Bruch's membrane complex and is another extremely prevalent cause of visual loss. We used mice in which the hypoxia response element (HRE) is deleted from the vascular endothelialg rowth factor(vegf) promoter (Vegf δ/δ mice) to explore the role of induction of VEGF through the HRE in these disease processes. Compared to wild type (Vegf δ/δ) mice with oxygen-induced ischemic retinopathy (OIR) in which vegf mRNA levels were increased and prominent retinal NV and BRB breakdown occurred, Vegfδ/δ littermates with OIR failed to increase vegf mRNA levels in the retina and had significantly less retinal NV and BRB breakdown, but showed prominent dilation of some superficial retinal vessels. Vegf+/δ littermates with ischemic retinopathy developed comparable retinal NV to Vegf+/+ mice, exhibited intermediate levels of BRB breakdown, and did not show vasodilation. In a mouse model of CNV, due to laser-induced rupture of Bruch's membrane, the area of CNV at Bruch's membrane rupture sites was more than tenfold greater in Vegf +/+ mice than in Vegfδ/δ littermates. In contrast to these dramatic differences in pathologic ocular NV, Vegf δ/δ mice showed subtle differences in retinal vascular development compared to Vegf+/+ mice; it was slightly delayed, but otherwise normal. These data suggest that induction of VEGF through the HRE in its promoter is critical for retinal and CNV, but not for retinal vascular development.
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U2 - 10.1002/jcp.20525
DO - 10.1002/jcp.20525
M3 - Article
C2 - 16245301
AN - SCOPUS:31944433624
SN - 0021-9541
VL - 206
SP - 749
EP - 758
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 3
ER -