Implication of protein carboxymethylation in retinal pigment epithelial cell chemotaxis

S. F. Hackett, P. A. Campochiaro

Research output: Contribution to journalArticlepeer-review

Abstract

Two chemoattractants for retinal pigment epithelial (RPE) cells, fibronectin (FN) and platelet-derived growth factor (PDGF) were found to enhance protein carboxymethylation mediated by S-adenosyl-L-methionine in RPE cells measured by [3H]methanol hydrolyzed from TCA precipitable protein methyl esters labelled with [3H]methionine. The effect was rapid, peaking at 2 min when [3H]methanol production was enhanced 120% by FN and 150% by PDGF. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA; 10 μM), adenosine (100 μM), and L-homocysteine thiolactone (100 μM) inhibited FN-induced carboxymethylation by 60%, and PDGF-induced carboxymethylation by 59%. In modified Boyden chamber assays, the combination of EHNA (10 μM), adenosine (100 μM), and L-homocysteine thiolactone (100 μM) markedly inhibited FN-induced chemotaxis by 88% and PDGF-induced chemotaxis by 93%. Migration of RPE cells has been implicated in the pathogenesis of proliferative vitreoretinopathy (PVR). Inhibition of protein carboxymethylation may provide a new target in the development of pharmacologic therapy for PVR.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalOphthalmic Research
Volume20
Issue number1
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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