Implication of protein carboxymethylation in retinal pigment epithelial cell chemotaxis

S. F. Hackett, Peter A Campochiaro

Research output: Contribution to journalArticle

Abstract

Two chemoattractants for retinal pigment epithelial (RPE) cells, fibronectin (FN) and platelet-derived growth factor (PDGF) were found to enhance protein carboxymethylation mediated by S-adenosyl-L-methionine in RPE cells measured by [3H]methanol hydrolyzed from TCA precipitable protein methyl esters labelled with [3H]methionine. The effect was rapid, peaking at 2 min when [3H]methanol production was enhanced 120% by FN and 150% by PDGF. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA; 10 μM), adenosine (100 μM), and L-homocysteine thiolactone (100 μM) inhibited FN-induced carboxymethylation by 60%, and PDGF-induced carboxymethylation by 59%. In modified Boyden chamber assays, the combination of EHNA (10 μM), adenosine (100 μM), and L-homocysteine thiolactone (100 μM) markedly inhibited FN-induced chemotaxis by 88% and PDGF-induced chemotaxis by 93%. Migration of RPE cells has been implicated in the pathogenesis of proliferative vitreoretinopathy (PVR). Inhibition of protein carboxymethylation may provide a new target in the development of pharmacologic therapy for PVR.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalOphthalmic Research
Volume20
Issue number1
StatePublished - 1988
Externally publishedYes

Fingerprint

Retinal Pigments
Platelet-Derived Growth Factor
Chemotaxis
Fibronectins
Epithelial Cells
Proliferative Vitreoretinopathy
Adenosine
Methanol
Proteins
S-Adenosylmethionine
Chemotactic Factors
Methionine
Esters
9-(2-hydroxy-3-nonyl)adenine
homocysteine thiolactone
Therapeutics

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Implication of protein carboxymethylation in retinal pigment epithelial cell chemotaxis. / Hackett, S. F.; Campochiaro, Peter A.

In: Ophthalmic Research, Vol. 20, No. 1, 1988, p. 54-59.

Research output: Contribution to journalArticle

@article{ff77f804157a4303bff428e0d6708470,
title = "Implication of protein carboxymethylation in retinal pigment epithelial cell chemotaxis",
abstract = "Two chemoattractants for retinal pigment epithelial (RPE) cells, fibronectin (FN) and platelet-derived growth factor (PDGF) were found to enhance protein carboxymethylation mediated by S-adenosyl-L-methionine in RPE cells measured by [3H]methanol hydrolyzed from TCA precipitable protein methyl esters labelled with [3H]methionine. The effect was rapid, peaking at 2 min when [3H]methanol production was enhanced 120{\%} by FN and 150{\%} by PDGF. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA; 10 μM), adenosine (100 μM), and L-homocysteine thiolactone (100 μM) inhibited FN-induced carboxymethylation by 60{\%}, and PDGF-induced carboxymethylation by 59{\%}. In modified Boyden chamber assays, the combination of EHNA (10 μM), adenosine (100 μM), and L-homocysteine thiolactone (100 μM) markedly inhibited FN-induced chemotaxis by 88{\%} and PDGF-induced chemotaxis by 93{\%}. Migration of RPE cells has been implicated in the pathogenesis of proliferative vitreoretinopathy (PVR). Inhibition of protein carboxymethylation may provide a new target in the development of pharmacologic therapy for PVR.",
author = "Hackett, {S. F.} and Campochiaro, {Peter A}",
year = "1988",
language = "English (US)",
volume = "20",
pages = "54--59",
journal = "Ophthalmic Research",
issn = "0030-3747",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Implication of protein carboxymethylation in retinal pigment epithelial cell chemotaxis

AU - Hackett, S. F.

AU - Campochiaro, Peter A

PY - 1988

Y1 - 1988

N2 - Two chemoattractants for retinal pigment epithelial (RPE) cells, fibronectin (FN) and platelet-derived growth factor (PDGF) were found to enhance protein carboxymethylation mediated by S-adenosyl-L-methionine in RPE cells measured by [3H]methanol hydrolyzed from TCA precipitable protein methyl esters labelled with [3H]methionine. The effect was rapid, peaking at 2 min when [3H]methanol production was enhanced 120% by FN and 150% by PDGF. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA; 10 μM), adenosine (100 μM), and L-homocysteine thiolactone (100 μM) inhibited FN-induced carboxymethylation by 60%, and PDGF-induced carboxymethylation by 59%. In modified Boyden chamber assays, the combination of EHNA (10 μM), adenosine (100 μM), and L-homocysteine thiolactone (100 μM) markedly inhibited FN-induced chemotaxis by 88% and PDGF-induced chemotaxis by 93%. Migration of RPE cells has been implicated in the pathogenesis of proliferative vitreoretinopathy (PVR). Inhibition of protein carboxymethylation may provide a new target in the development of pharmacologic therapy for PVR.

AB - Two chemoattractants for retinal pigment epithelial (RPE) cells, fibronectin (FN) and platelet-derived growth factor (PDGF) were found to enhance protein carboxymethylation mediated by S-adenosyl-L-methionine in RPE cells measured by [3H]methanol hydrolyzed from TCA precipitable protein methyl esters labelled with [3H]methionine. The effect was rapid, peaking at 2 min when [3H]methanol production was enhanced 120% by FN and 150% by PDGF. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA; 10 μM), adenosine (100 μM), and L-homocysteine thiolactone (100 μM) inhibited FN-induced carboxymethylation by 60%, and PDGF-induced carboxymethylation by 59%. In modified Boyden chamber assays, the combination of EHNA (10 μM), adenosine (100 μM), and L-homocysteine thiolactone (100 μM) markedly inhibited FN-induced chemotaxis by 88% and PDGF-induced chemotaxis by 93%. Migration of RPE cells has been implicated in the pathogenesis of proliferative vitreoretinopathy (PVR). Inhibition of protein carboxymethylation may provide a new target in the development of pharmacologic therapy for PVR.

UR - http://www.scopus.com/inward/record.url?scp=0023840971&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023840971&partnerID=8YFLogxK

M3 - Article

C2 - 3380525

AN - SCOPUS:0023840971

VL - 20

SP - 54

EP - 59

JO - Ophthalmic Research

JF - Ophthalmic Research

SN - 0030-3747

IS - 1

ER -