Implementation of a semiclosed large scale counterflow centrifugal elutriation system

Ino K. Gao, Stephen J. Noga, John E. Wagner, Carol A. Cremo, Janice Davis, Albert D. Donnenberg

Research output: Contribution to journalArticlepeer-review

Abstract

A semiclosed counterflow centrifugal elutriation system that minimizes the risks of contamination and cell loss is presented. A detailed description of the configuration of the system and its sterilization and assembly is provided along with examples of its application to large‐scale separation of leukapheresis buffy coat and cadaveric bone marrow. We are currently using this system to deplete lymphocytes from human bone marrow prior to use in allogeneic bone marrow transplantation. The implementation of a semiclosed system increases the safety, ease of operation, and reproduciblity of a technique that has the potential for a wide range of clinical applications.

Original languageEnglish (US)
Pages (from-to)154-160
Number of pages7
JournalJournal of Clinical Apheresis
Volume3
Issue number3
DOIs
StatePublished - 1987

Keywords

  • Beckman JE‐10X Rotor
  • bone marrow transplantation
  • cell separation
  • closed system
  • human bone marrow

ASJC Scopus subject areas

  • Hematology

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