Abstract
Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. The long-term clinical results showed excellent patency rates, however, with significant incidence of stenosis. To investigate the cellular and molecular mechanisms of vascular neotissue formation and prevent stenosis development in tissue engineered vascular grafts (TEVGs), we developed a mouse model of the graft with approximately 1 mm internal diameter. First, the TEVGs were assembled from biodegradable tubular scaffolds fabricated from a polyglycolic acid nonwoven felt mesh coated with ε-caprolactone and L-lactide copolymer. The scaffolds were then placed in a lyophilizer, vacuumed for 24 hr, and stored in a desiccator until cell seeding. Second, bone marrow was collected from donor mice and mononuclear cells were isolated by density gradient centrifugation Third, approximately one million cells were seeded on a scaffold and incubated O/N. Finally, the seeded scaffolds were then implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. The implanted grafts demonstrated excellent patency (>90%) without evidence of thromboembolic complications or aneurysmal formation. This murine model will aid us in understanding and quantifying the cellular and molecular mechanisms of neotissue formation in the TEVG.
| Original language | English (US) |
|---|---|
| Article number | e51632 |
| Journal | Journal of Visualized Experiments |
| Issue number | 88 |
| DOIs | |
| State | Published - Jun 4 2014 |
| Externally published | Yes |
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Keywords
- Biodegradable
- Inferior vena cava
- Interposition graft
- Issue 88
- Medicine
- Mouse model
- Tissue engineered vascular graft
- Tissue engineering
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Chemical Engineering(all)
- Immunology and Microbiology(all)
- Neuroscience(all)
Cite this
Implantation of inferior vena cava interposition graft in mouse model. / Lee, Yong Ung; Yi, Tai; Tara, Shuhei; Lee, Avione Y.; Hibino, Narutoshi; Shinoka, Toshiharu; Breuer, Christopher K.
In: Journal of Visualized Experiments, No. 88, e51632, 04.06.2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Implantation of inferior vena cava interposition graft in mouse model
AU - Lee, Yong Ung
AU - Yi, Tai
AU - Tara, Shuhei
AU - Lee, Avione Y.
AU - Hibino, Narutoshi
AU - Shinoka, Toshiharu
AU - Breuer, Christopher K.
PY - 2014/6/4
Y1 - 2014/6/4
N2 - Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. The long-term clinical results showed excellent patency rates, however, with significant incidence of stenosis. To investigate the cellular and molecular mechanisms of vascular neotissue formation and prevent stenosis development in tissue engineered vascular grafts (TEVGs), we developed a mouse model of the graft with approximately 1 mm internal diameter. First, the TEVGs were assembled from biodegradable tubular scaffolds fabricated from a polyglycolic acid nonwoven felt mesh coated with ε-caprolactone and L-lactide copolymer. The scaffolds were then placed in a lyophilizer, vacuumed for 24 hr, and stored in a desiccator until cell seeding. Second, bone marrow was collected from donor mice and mononuclear cells were isolated by density gradient centrifugation Third, approximately one million cells were seeded on a scaffold and incubated O/N. Finally, the seeded scaffolds were then implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. The implanted grafts demonstrated excellent patency (>90%) without evidence of thromboembolic complications or aneurysmal formation. This murine model will aid us in understanding and quantifying the cellular and molecular mechanisms of neotissue formation in the TEVG.
AB - Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. The long-term clinical results showed excellent patency rates, however, with significant incidence of stenosis. To investigate the cellular and molecular mechanisms of vascular neotissue formation and prevent stenosis development in tissue engineered vascular grafts (TEVGs), we developed a mouse model of the graft with approximately 1 mm internal diameter. First, the TEVGs were assembled from biodegradable tubular scaffolds fabricated from a polyglycolic acid nonwoven felt mesh coated with ε-caprolactone and L-lactide copolymer. The scaffolds were then placed in a lyophilizer, vacuumed for 24 hr, and stored in a desiccator until cell seeding. Second, bone marrow was collected from donor mice and mononuclear cells were isolated by density gradient centrifugation Third, approximately one million cells were seeded on a scaffold and incubated O/N. Finally, the seeded scaffolds were then implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. The implanted grafts demonstrated excellent patency (>90%) without evidence of thromboembolic complications or aneurysmal formation. This murine model will aid us in understanding and quantifying the cellular and molecular mechanisms of neotissue formation in the TEVG.
KW - Biodegradable
KW - Inferior vena cava
KW - Interposition graft
KW - Issue 88
KW - Medicine
KW - Mouse model
KW - Tissue engineered vascular graft
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=84929789491&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84929789491&partnerID=8YFLogxK
U2 - 10.3791/51632
DO - 10.3791/51632
M3 - Article
C2 - 24961688
AN - SCOPUS:84929789491
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
SN - 1940-087X
IS - 88
M1 - e51632
ER -