Impedimetric fingerprinting and structural analysis of isogenic E. coli biofilms using multielectrode arrays

Erkuden Goikoetxea, Denis Routkevitch, Ami de Weerdt, Jordan Green, Hans Steenackers, Dries Braeken

Research output: Contribution to journalArticle

Abstract

Microbial biofilm contamination is an ubiquitous and persistent problem in industry and clinics. The structure of the biofilm, its extracellular matrix and its formation process are very complex. At present, there are only limited options to investigate biofilms outside the lab, as most in situ techniques lack sensitivity and resolution. Impedance-based sensors provide a fast, label-free and sensitive manner to characterize biofilms, although mainly large electrodes have been used so far. Here, we used 60 μm-sized electrode arrays (MEAs) to characterize the structure of biofilms formed by wild type (WT) Escherichia coli TG1 and the isogenic ΔcsgD, ΔcsgB and ΔbcsA mutants. At 24 h of growth, the interfacial resistance at 2 Hz increased by 3.4% and 0.3% for the curli producing strains (WT and ΔbcsA), yet it decreased by 5.7% and 4% for the curli non-producing strains (ΔcsgD and ΔcsgB). The imaginary impedance at 2 Hz decreased for all the strains by 7.2%, 6.9%, 5.1% and 2.5% (WT, ΔbcsA, ΔcsgB and ΔcsgD, respectively). Interestingly, the spatial variation of impedance within each biofilm, resulting from physiological and structural heterogeneity, was significantly different for each biofilm and most pronounced in the WT. Depending on the strain, the biofilm attachment phase lasted between 6 and 10 h, and was characterized by an increase in the interfacial resistance of up to 6% for the WT, 5.5% for ΔcsgD, 3.5% for ΔcsgB and 5% for ΔbcsA, as opposed to the decrease in medium resistance observed during the maturation phase. Overall, impedance-based MEA assays proved effective to differentiate between biofilms with varying structure, detect spatial diversity and explain biofilm life-cycle in terms of attachment and maturation.

Original languageEnglish (US)
Pages (from-to)319-326
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume263
DOIs
StatePublished - Jun 15 2018

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biofilms
Biofilms
structural analysis
Structural analysis
Escherichia coli
impedance
Electrodes
attachment
electrodes
Escherichia
Labels
Life cycle
Assays
contamination
Contamination
industries

Keywords

  • Bacterial biofilms
  • Electrochemical impedance spectroscopy
  • Label free detection
  • Microelectrode arrays

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Impedimetric fingerprinting and structural analysis of isogenic E. coli biofilms using multielectrode arrays. / Goikoetxea, Erkuden; Routkevitch, Denis; de Weerdt, Ami; Green, Jordan; Steenackers, Hans; Braeken, Dries.

In: Sensors and Actuators, B: Chemical, Vol. 263, 15.06.2018, p. 319-326.

Research output: Contribution to journalArticle

Goikoetxea, Erkuden ; Routkevitch, Denis ; de Weerdt, Ami ; Green, Jordan ; Steenackers, Hans ; Braeken, Dries. / Impedimetric fingerprinting and structural analysis of isogenic E. coli biofilms using multielectrode arrays. In: Sensors and Actuators, B: Chemical. 2018 ; Vol. 263. pp. 319-326.
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