Impaired Skeletal Muscle Regeneration in the Absence of Fibrosis during Hibernation in 13-Lined Ground Squirrels

Eva Andres-Mateos, Rebeca Mejias, Arshia Soleimani, Brian M. Lin, Tyesha N. Burks, Ruth Marx-Rattner, Benjamin Lin, Richard C. Zellars, Yonggang Zhang, David L. Huso, Tom G. Marr, Leslie A. Leinwand, Dana K. Merriman, Ronald D. Cohn

Research output: Contribution to journalArticle

Abstract

Skeletal muscle atrophy can occur as a consequence of immobilization and/or starvation in the majority of vertebrates studied. In contrast, hibernating mammals are protected against the loss of muscle mass despite long periods of inactivity and lack of food intake. Resident muscle-specific stem cells (satellite cells) are known to be activated by muscle injury and their activation contributes to the regeneration of muscle, but whether satellite cells play a role in hibernation is unknown. In the hibernating 13-lined ground squirrel we show that muscles ablated of satellite cells were still protected against atrophy, demonstrating that satellite cells are not involved in the maintenance of skeletal muscle during hibernation. Additionally, hibernating skeletal muscle showed extremely slow regeneration in response to injury, due to repression of satellite cell activation and myoblast differentiation caused by a fine-tuned interplay of p21, myostatin, MAPK, and Wnt signaling pathways. Interestingly, despite long periods of inflammation and lack of efficient regeneration, injured skeletal muscle from hibernating animals did not develop fibrosis and was capable of complete recovery when animals emerged naturally from hibernation. We propose that hibernating squirrels represent a new model system that permits evaluation of impaired skeletal muscle remodeling in the absence of formation of tissue fibrosis.

Original languageEnglish (US)
Article numbere48884
JournalPLoS One
Volume7
Issue number11
DOIs
StatePublished - Nov 14 2012

Fingerprint

Hibernation
Sciuridae
muscle development
squirrels
hibernation
fibrosis
Muscle
skeletal muscle
Regeneration
Skeletal Muscle
Fibrosis
Muscles
muscles
Satellites
cells
Myostatin
myostatin
muscular atrophy
myoblasts
Wnt Signaling Pathway

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Impaired Skeletal Muscle Regeneration in the Absence of Fibrosis during Hibernation in 13-Lined Ground Squirrels. / Andres-Mateos, Eva; Mejias, Rebeca; Soleimani, Arshia; Lin, Brian M.; Burks, Tyesha N.; Marx-Rattner, Ruth; Lin, Benjamin; Zellars, Richard C.; Zhang, Yonggang; Huso, David L.; Marr, Tom G.; Leinwand, Leslie A.; Merriman, Dana K.; Cohn, Ronald D.

In: PLoS One, Vol. 7, No. 11, e48884, 14.11.2012.

Research output: Contribution to journalArticle

Andres-Mateos, E, Mejias, R, Soleimani, A, Lin, BM, Burks, TN, Marx-Rattner, R, Lin, B, Zellars, RC, Zhang, Y, Huso, DL, Marr, TG, Leinwand, LA, Merriman, DK & Cohn, RD 2012, 'Impaired Skeletal Muscle Regeneration in the Absence of Fibrosis during Hibernation in 13-Lined Ground Squirrels', PLoS One, vol. 7, no. 11, e48884. https://doi.org/10.1371/journal.pone.0048884
Andres-Mateos, Eva ; Mejias, Rebeca ; Soleimani, Arshia ; Lin, Brian M. ; Burks, Tyesha N. ; Marx-Rattner, Ruth ; Lin, Benjamin ; Zellars, Richard C. ; Zhang, Yonggang ; Huso, David L. ; Marr, Tom G. ; Leinwand, Leslie A. ; Merriman, Dana K. ; Cohn, Ronald D. / Impaired Skeletal Muscle Regeneration in the Absence of Fibrosis during Hibernation in 13-Lined Ground Squirrels. In: PLoS One. 2012 ; Vol. 7, No. 11.
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