Impaired reproduction in transgenic mice overexpressingγ-aminobutyric acid transporter I (GAT1)

Jia Hua Hu, Jin Fu Zhang, Ying Hua Ma, Jie Jiang, Na Yang, Xin Bo Li, Zhi Guang Yu Chi, Jian Fei, Li He Guo

Research output: Contribution to journalArticle

Abstract

It is well documented that γ-aminobutyric acid (GABA) system existed in reproductive organs. Recent researches showed that GABAA and GABAB receptors were present in testis and sperm, and might mediate the acrosome reaction induced by GABA and progesterone. GABA transporter I (GAT1) also existed in testis and sperm, but its physiological function was unknown. In the present study, we used GAT1 overexpressing mice to explore GAT1 function in male reproductive system. We found that the expression level of GAT1 continuously increased in wild-type mouse testis from 1 month to 2 months after birth. GAT1 overexpression in mouse affected testis development, which embodied reduced testis mass and slowed spermatogenesis in transgenic mice. Moreover, transgenic mice showed increase of the percentage of broken sperm. The further study revealed that the reproductive capacity was impaired in GAT1 overexpressing mice. In addition, testosterone level was significantly low in transgenic mice compared with that in wild-type mice. Our findings provided the first evidence that abnormal expression of GAT1 could result in dysgenesis, and indicated that GAT1 might be therapeutically targeted for contraception or dysgenesis treatment.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalCell Research
Volume14
Issue number1
DOIs
StatePublished - Feb 2004
Externally publishedYes

Fingerprint

GABA Plasma Membrane Transport Proteins
Aminobutyrates
Transgenic Mice
Reproduction
Testis
Spermatozoa
gamma-Aminobutyric Acid
Acrosome Reaction
GABA-A Receptors
Spermatogenesis
Contraception
Progesterone
Testosterone
Parturition
Research

Keywords

  • GABA
  • GAT1
  • Reproduction
  • Sperm
  • Testes
  • Transgenic

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hu, J. H., Zhang, J. F., Ma, Y. H., Jiang, J., Yang, N., Li, X. B., ... Guo, L. H. (2004). Impaired reproduction in transgenic mice overexpressingγ-aminobutyric acid transporter I (GAT1). Cell Research, 14(1), 54-59. https://doi.org/10.1038/sj.cr.7290202

Impaired reproduction in transgenic mice overexpressingγ-aminobutyric acid transporter I (GAT1). / Hu, Jia Hua; Zhang, Jin Fu; Ma, Ying Hua; Jiang, Jie; Yang, Na; Li, Xin Bo; Yu Chi, Zhi Guang; Fei, Jian; Guo, Li He.

In: Cell Research, Vol. 14, No. 1, 02.2004, p. 54-59.

Research output: Contribution to journalArticle

Hu, JH, Zhang, JF, Ma, YH, Jiang, J, Yang, N, Li, XB, Yu Chi, ZG, Fei, J & Guo, LH 2004, 'Impaired reproduction in transgenic mice overexpressingγ-aminobutyric acid transporter I (GAT1)', Cell Research, vol. 14, no. 1, pp. 54-59. https://doi.org/10.1038/sj.cr.7290202
Hu, Jia Hua ; Zhang, Jin Fu ; Ma, Ying Hua ; Jiang, Jie ; Yang, Na ; Li, Xin Bo ; Yu Chi, Zhi Guang ; Fei, Jian ; Guo, Li He. / Impaired reproduction in transgenic mice overexpressingγ-aminobutyric acid transporter I (GAT1). In: Cell Research. 2004 ; Vol. 14, No. 1. pp. 54-59.
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