Impaired postprandial releases/syntheses of ghrelin and PYY3-36 and blunted responses to exogenous ghrelin and PYY3-36 in a rodent model of diet-induced obesity

Junying Xu, Terry A. Mcnearney, Jiande Chen

Research output: Contribution to journalArticle

Abstract

Background and Aim: This study investigated the effects of peripheral administration of ghrelin and PYY3-36 on food intake and plasma and tissue fasting and postprandial ghrelin and PYY3-36 levels in normal-weight (NW) and diet-induced-obese (DIO) rats. Methods: In experiment one, NW and DIO rats received a single intraperitoneal injection of saline, PYY3-36 or ghrelin; food intake was measured for 4h. In experiment two, total plasma ghrelin and PYY3-36, gastric fundus ghrelin, and ascending colon PYY3-36 were measured either after a 20-h fast or 2h after refeeding in NW and DIO rats by radioimmunoassay. Results: Compared to the NW rats, findings in the DIO rats revealed: (i) a reduced sensitivity to both the anorectic effect of exogenous PYY3-36 and the orexigenic effect of exogenous ghrelin; (ii) the postprandial plasma ghrelin levels were significantly higher; and (iii) refeeding decreased endogenous plasma ghrelin levels by 53% in the NW rats and 39% in DIO rats. Refeeding increased the plasma PYY3-36 level by 58% in the NW rats versus 9% in the DIO rats (P=0.003). Conclusions: Compared with regular rats, DIO rats exhibit blunted responses in food intake to exogenous ghrelin and PYY3-36. Although endogenous ghrelin and PYY3-36 in DIO rats are not altered in the fasting state, their responses to food ingestion are blunted in comparison with regular rats.

Original languageEnglish (US)
Pages (from-to)700-705
Number of pages6
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume26
Issue number4
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

Fingerprint

Ghrelin
Rodentia
Obesity
Diet
Weights and Measures
Eating
Fasting
Gastric Fundus
Ascending Colon
Appetite Depressants
Intraperitoneal Injections
Radioimmunoassay

Keywords

  • Diet-induced obesity
  • Food response
  • Ghrelin
  • PYY

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

@article{ff94a8d166f54b8985bcd574912f62f0,
title = "Impaired postprandial releases/syntheses of ghrelin and PYY3-36 and blunted responses to exogenous ghrelin and PYY3-36 in a rodent model of diet-induced obesity",
abstract = "Background and Aim: This study investigated the effects of peripheral administration of ghrelin and PYY3-36 on food intake and plasma and tissue fasting and postprandial ghrelin and PYY3-36 levels in normal-weight (NW) and diet-induced-obese (DIO) rats. Methods: In experiment one, NW and DIO rats received a single intraperitoneal injection of saline, PYY3-36 or ghrelin; food intake was measured for 4h. In experiment two, total plasma ghrelin and PYY3-36, gastric fundus ghrelin, and ascending colon PYY3-36 were measured either after a 20-h fast or 2h after refeeding in NW and DIO rats by radioimmunoassay. Results: Compared to the NW rats, findings in the DIO rats revealed: (i) a reduced sensitivity to both the anorectic effect of exogenous PYY3-36 and the orexigenic effect of exogenous ghrelin; (ii) the postprandial plasma ghrelin levels were significantly higher; and (iii) refeeding decreased endogenous plasma ghrelin levels by 53{\%} in the NW rats and 39{\%} in DIO rats. Refeeding increased the plasma PYY3-36 level by 58{\%} in the NW rats versus 9{\%} in the DIO rats (P=0.003). Conclusions: Compared with regular rats, DIO rats exhibit blunted responses in food intake to exogenous ghrelin and PYY3-36. Although endogenous ghrelin and PYY3-36 in DIO rats are not altered in the fasting state, their responses to food ingestion are blunted in comparison with regular rats.",
keywords = "Diet-induced obesity, Food response, Ghrelin, PYY",
author = "Junying Xu and Mcnearney, {Terry A.} and Jiande Chen",
year = "2011",
month = "1",
day = "1",
doi = "10.1111/j.1440-1746.2010.06563.x",
language = "English (US)",
volume = "26",
pages = "700--705",
journal = "Journal of Gastroenterology and Hepatology (Australia)",
issn = "0815-9319",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Impaired postprandial releases/syntheses of ghrelin and PYY3-36 and blunted responses to exogenous ghrelin and PYY3-36 in a rodent model of diet-induced obesity

AU - Xu, Junying

AU - Mcnearney, Terry A.

AU - Chen, Jiande

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Background and Aim: This study investigated the effects of peripheral administration of ghrelin and PYY3-36 on food intake and plasma and tissue fasting and postprandial ghrelin and PYY3-36 levels in normal-weight (NW) and diet-induced-obese (DIO) rats. Methods: In experiment one, NW and DIO rats received a single intraperitoneal injection of saline, PYY3-36 or ghrelin; food intake was measured for 4h. In experiment two, total plasma ghrelin and PYY3-36, gastric fundus ghrelin, and ascending colon PYY3-36 were measured either after a 20-h fast or 2h after refeeding in NW and DIO rats by radioimmunoassay. Results: Compared to the NW rats, findings in the DIO rats revealed: (i) a reduced sensitivity to both the anorectic effect of exogenous PYY3-36 and the orexigenic effect of exogenous ghrelin; (ii) the postprandial plasma ghrelin levels were significantly higher; and (iii) refeeding decreased endogenous plasma ghrelin levels by 53% in the NW rats and 39% in DIO rats. Refeeding increased the plasma PYY3-36 level by 58% in the NW rats versus 9% in the DIO rats (P=0.003). Conclusions: Compared with regular rats, DIO rats exhibit blunted responses in food intake to exogenous ghrelin and PYY3-36. Although endogenous ghrelin and PYY3-36 in DIO rats are not altered in the fasting state, their responses to food ingestion are blunted in comparison with regular rats.

AB - Background and Aim: This study investigated the effects of peripheral administration of ghrelin and PYY3-36 on food intake and plasma and tissue fasting and postprandial ghrelin and PYY3-36 levels in normal-weight (NW) and diet-induced-obese (DIO) rats. Methods: In experiment one, NW and DIO rats received a single intraperitoneal injection of saline, PYY3-36 or ghrelin; food intake was measured for 4h. In experiment two, total plasma ghrelin and PYY3-36, gastric fundus ghrelin, and ascending colon PYY3-36 were measured either after a 20-h fast or 2h after refeeding in NW and DIO rats by radioimmunoassay. Results: Compared to the NW rats, findings in the DIO rats revealed: (i) a reduced sensitivity to both the anorectic effect of exogenous PYY3-36 and the orexigenic effect of exogenous ghrelin; (ii) the postprandial plasma ghrelin levels were significantly higher; and (iii) refeeding decreased endogenous plasma ghrelin levels by 53% in the NW rats and 39% in DIO rats. Refeeding increased the plasma PYY3-36 level by 58% in the NW rats versus 9% in the DIO rats (P=0.003). Conclusions: Compared with regular rats, DIO rats exhibit blunted responses in food intake to exogenous ghrelin and PYY3-36. Although endogenous ghrelin and PYY3-36 in DIO rats are not altered in the fasting state, their responses to food ingestion are blunted in comparison with regular rats.

KW - Diet-induced obesity

KW - Food response

KW - Ghrelin

KW - PYY

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U2 - 10.1111/j.1440-1746.2010.06563.x

DO - 10.1111/j.1440-1746.2010.06563.x

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VL - 26

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JO - Journal of Gastroenterology and Hepatology (Australia)

JF - Journal of Gastroenterology and Hepatology (Australia)

SN - 0815-9319

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