Impaired expression of thrombospondin-1 in eyes with age related macular degeneration

K. Uno, I. A. Bhutto, D. S. McLeod, C. Merges, Gerard Anthony Lutty

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Aims: This study investigated the expression and localisation of thrombospondin-1 (TSP-1 ), a known antiangiogenic extracellular matrix protein, in normal aged control human eyes and eyes with age related macular degeneration (AMD). Methods: Immunohistochemical analysis with mouse anti-human TSP-1 antibody and mouse anti-human CD 34 antibody, as a blood vessel marker, was performed on frozen sections from macular and peripheral blocks of aged control donor eyes (n = 12; mean age 78.8 years), and eyes with AMD (n = 12; mean age 83.9 years). Pigment in retinal pigment epithelium (RPE) and choroidal melanocytes was bleached. Three independent observers scored the immunohistochemical reaction product. Results: In the macular region, TSP-1 expression was observed intensely in Bruch's membrane and weakly in RPE basement membrane, choriocapillaris, and the wall of large choroidal blood vessels in the aged control eyes. In eyes with AMD, TSP-1 immunoreactivity was significantly lower in all structures except RPE basement membrane (p

Original languageEnglish (US)
Pages (from-to)48-54
Number of pages7
JournalBritish Journal of Ophthalmology
Issue number1
Publication statusPublished - Jan 2006


ASJC Scopus subject areas

  • Ophthalmology

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