Impacts of interleukin-12 on multiplication of Mycobacterium tuberculosis and Mycobacterium avium complex in mice

Nacer Lounis, Baohong Ji, Chantal Truffot-Pernot, Robert G. Ridley, Gottfried Alber, Jacques H. Grosset

Research output: Contribution to journalArticle

Abstract

Objective: To evaluate the potential impacts of exogenous administration of murine recombinant interleukin-12 (IL-12) on multiplication of Mycobacterium tuberculosis and M. avium complex (MAC) in murine models. Methods: Swiss or beige mice were infected intravenously with M. tuberculosis H37Rv or MAC respectively, and were treated by subcutaneous injection with various doses of IL-12, either alone or in combination with chemotherapy. Effectiveness of treatment was assessed by the enumeration of CFUs in the spleens and lungs, together with other indicators. Results: Multiplication of M. tuberculosis was reduced by IL-12 in a dose-dependent manner if the treatment began at day 1, whereas no statistically significant suppression was observed if the treatment began at day 14. Combination with IL-12 did not enhance the bactericidal activity of antituberculosis chemotherapy. The growth curves of MAC in IL-12-treated mice were almost identical to those of untreated controls, indicating that IL-12 did not affect the multiplication of MAC in beige mice. In both experiments, the dosing of IL-12 approached levels of severe toxicity for the mouse strains used. Conclusions: IL-12 had a positive affect on early multiplication of M. tuberculosis. It had no effect on early multiplication of M. avium complex.

Original languageEnglish (US)
Pages (from-to)331-338
Number of pages8
JournalClinical Microbiology and Infection
Volume5
Issue number6
StatePublished - Jun 1999
Externally publishedYes

Fingerprint

Mycobacterium avium Complex
Interleukin-12
Mycobacterium tuberculosis
Subcutaneous Injections
Combination Drug Therapy
Spleen
Drug Therapy
Lung
Therapeutics
Growth

Keywords

  • Mycobacterium avium complex
  • Mycobacterium tuberculosis
  • Recombinant interleukin-12

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Lounis, N., Ji, B., Truffot-Pernot, C., Ridley, R. G., Alber, G., & Grosset, J. H. (1999). Impacts of interleukin-12 on multiplication of Mycobacterium tuberculosis and Mycobacterium avium complex in mice. Clinical Microbiology and Infection, 5(6), 331-338.

Impacts of interleukin-12 on multiplication of Mycobacterium tuberculosis and Mycobacterium avium complex in mice. / Lounis, Nacer; Ji, Baohong; Truffot-Pernot, Chantal; Ridley, Robert G.; Alber, Gottfried; Grosset, Jacques H.

In: Clinical Microbiology and Infection, Vol. 5, No. 6, 06.1999, p. 331-338.

Research output: Contribution to journalArticle

Lounis, N, Ji, B, Truffot-Pernot, C, Ridley, RG, Alber, G & Grosset, JH 1999, 'Impacts of interleukin-12 on multiplication of Mycobacterium tuberculosis and Mycobacterium avium complex in mice', Clinical Microbiology and Infection, vol. 5, no. 6, pp. 331-338.
Lounis, Nacer ; Ji, Baohong ; Truffot-Pernot, Chantal ; Ridley, Robert G. ; Alber, Gottfried ; Grosset, Jacques H. / Impacts of interleukin-12 on multiplication of Mycobacterium tuberculosis and Mycobacterium avium complex in mice. In: Clinical Microbiology and Infection. 1999 ; Vol. 5, No. 6. pp. 331-338.
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