@article{789635dd1a654a60986230e79610445e,
title = "Impact of regulated secretion on antiparasitic CD8 T cell responses",
abstract = "CD8 Tcells play a key role in defense against the intracellular parasite Toxoplasma, but why certain CD8 responses are more potent than others is not well understood. Here, we describe a parasite antigen, ROP5, that elicits a CD8 Tcell response in genetically susceptible mice. ROP5 is secreted via parasite organelles termed rhoptries that are injected directly into host cells during invasion, whereas the protective, dense-granule antigen GRA6 is constitutively secreted into the parasitophorous vacuole. Transgenic parasites in which the ROP5 antigenic epitope was targeted for secretion through dense granules led to enhanced CD8 Tcell responses, whereas targeting the GRA6 epitope to rhoptries led to reduced CD8 responses. CD8 Tcell responses to the dense-granule-targeted ROP5 epitope resulted in reduced parasite load in the brain. These data suggest that the mode of secretion affects the efficacy of parasite-specific CD8 Tcell responses.",
author = "Grover, {Harshita Satija} and Chu, {H. Hamlet} and Kelly, {Felice D.} and Yang, {Soo Jung} and Reese, {Michael L.} and Nicolas Blanchard and Federico Gonzalez and Chan, {Shiao Wei} and Boothroyd, {John C.} and Nilabh Shastri and Robey, {Ellen A.}",
note = "Funding Information: We thank Jon Boyle for access to unpublished information prior to publication and Kerry Buchholz for technical assistance and discussion of results. We are grateful to the NIH Tetramer Facility for the YAL9-D b and the HF10-L d tetramers, and the Cancer Research Laboratory Flow Facility at UC Berkeley for technical assistance. Imaging for immunofluorescence analysis was performed at the Stanford Neuroscience Microscopy Service, supported by NIH NS069375. This work was supported by NIH grants P01 AI065831 to E.A.R. and N.S., R01 AI065537 to E.A.R., and R01 AI076753 to J.C.B. H.S.G. was supported in part by a Cancer Research Coordinating Committee fellowship. M.L.R. was supported by an American Cancer Society fellowship. H.C. was supported by an American Heart Association postdoctoral fellowship. F.K. was supported by an NIH F32 fellowship. ",
year = "2014",
month = jun,
day = "12",
doi = "10.1016/j.celrep.2014.04.031",
language = "English (US)",
volume = "7",
pages = "1716--1728",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",
}