Impact of nasopharyngeal swab types on detection of Bordetella pertussis by PCR and culture

Joann L. Cloud; Weston Hymas; Karen C. Carroll

doi:10.1128/JCM.40.10.3838-3840.2002

Impact of nasopharyngeal swab types on detection of Bordetella pertussis by PCR and culture

Joann L. Cloud, Weston Hymas, Karen C. Carroll

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

A study was designed to assess the performance of various swabs and transport media routinely used to collect specimens submitted for Bordetella culture and PCR. Calcium-alginate swabs inhibited the PCR. No inhibition was detected in any PCRs with dacron or rayon swabs. All swab materials performed similarly for recovery of Bordetella pertussis in culture. The Amies with charcoal transport system performed poorly for culture. Calcium-alginate swabs are not recommended for PCR-based detection of B. pertussis. Dacron and rayon swabs are an excellent choice for both PCR and culture.

Original language	English (US)
Pages (from-to)	3838-3840
Number of pages	3
Journal	Journal of clinical microbiology
Volume	40
Issue number	10
DOIs	https://doi.org/10.1128/JCM.40.10.3838-3840.2002
State	Published - Oct 1 2002
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.40.10.3838-3840.2002

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abstract = "A study was designed to assess the performance of various swabs and transport media routinely used to collect specimens submitted for Bordetella culture and PCR. Calcium-alginate swabs inhibited the PCR. No inhibition was detected in any PCRs with dacron or rayon swabs. All swab materials performed similarly for recovery of Bordetella pertussis in culture. The Amies with charcoal transport system performed poorly for culture. Calcium-alginate swabs are not recommended for PCR-based detection of B. pertussis. Dacron and rayon swabs are an excellent choice for both PCR and culture.",

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AB - A study was designed to assess the performance of various swabs and transport media routinely used to collect specimens submitted for Bordetella culture and PCR. Calcium-alginate swabs inhibited the PCR. No inhibition was detected in any PCRs with dacron or rayon swabs. All swab materials performed similarly for recovery of Bordetella pertussis in culture. The Amies with charcoal transport system performed poorly for culture. Calcium-alginate swabs are not recommended for PCR-based detection of B. pertussis. Dacron and rayon swabs are an excellent choice for both PCR and culture.

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Impact of nasopharyngeal swab types on detection of Bordetella pertussis by PCR and culture

Abstract

ASJC Scopus subject areas

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