Immunoradiometric assay to measure the in vitro penetration of sporozoites of malaria parasites into hepatoma cells

Fidel P Zavala, M. R. Hollingdale, A. L. Schwartz, R. S. Nussenzweig, V. Nussenzweig

Research output: Contribution to journalArticle

Abstract

We describe here an immunoradiometric assay to quantitate the in vitro invasion of hepatoma cells by sporozoites. The assay measures levels of circumsporozoite (CS) antigen that remain associated with the hepatoma cells after their incubation with the parasites. Several observations show that these measurements reflect internalized rather than extracellular antigen. For example, when incubations were performed with nonviable parasites (sonicated or heated), or in the presence of metabolic inhibitors, such as sodium azide and deoxyglucose, the amounts of CS antigen found in hepatoma cell extracts were greatly diminished. Moreover, Western blotting experiments revealed a striking difference in the pattern of CS proteins of infected cell extracts as compared with those of free parasites. The assay was used to measure the amounts of intracellular CS antigen for several days after infection of the hepatoma cells. The results confirmed previous microscopic observations, made by using immunofluorescence techniques, showing that the CS antigen in the host's liver cells diminishes progressively while the parasite develops into the exoerythrocytic stage. The immunoradiometric assay should facilitate the evaluation of the effects of drugs on sporozoites and also on studies aimed at the identification of a sporozoite receptor on the hepatocyte.

Original languageEnglish (US)
Pages (from-to)1202-1205
Number of pages4
JournalJournal of Immunology
Volume134
Issue number2
StatePublished - 1985
Externally publishedYes

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Immunoradiometric Assay
Sporozoites
Malaria
Hepatocellular Carcinoma
Parasites
Antigens
Cell Extracts
Sodium Azide
Drug Evaluation
Deoxyglucose
Fluorescent Antibody Technique
Hepatocytes
Western Blotting
In Vitro Techniques
Liver
Infection
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Zavala, F. P., Hollingdale, M. R., Schwartz, A. L., Nussenzweig, R. S., & Nussenzweig, V. (1985). Immunoradiometric assay to measure the in vitro penetration of sporozoites of malaria parasites into hepatoma cells. Journal of Immunology, 134(2), 1202-1205.

Immunoradiometric assay to measure the in vitro penetration of sporozoites of malaria parasites into hepatoma cells. / Zavala, Fidel P; Hollingdale, M. R.; Schwartz, A. L.; Nussenzweig, R. S.; Nussenzweig, V.

In: Journal of Immunology, Vol. 134, No. 2, 1985, p. 1202-1205.

Research output: Contribution to journalArticle

Zavala, FP, Hollingdale, MR, Schwartz, AL, Nussenzweig, RS & Nussenzweig, V 1985, 'Immunoradiometric assay to measure the in vitro penetration of sporozoites of malaria parasites into hepatoma cells', Journal of Immunology, vol. 134, no. 2, pp. 1202-1205.
Zavala, Fidel P ; Hollingdale, M. R. ; Schwartz, A. L. ; Nussenzweig, R. S. ; Nussenzweig, V. / Immunoradiometric assay to measure the in vitro penetration of sporozoites of malaria parasites into hepatoma cells. In: Journal of Immunology. 1985 ; Vol. 134, No. 2. pp. 1202-1205.
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