Immunoradiometric assay for detection of filarial antigens in human serum

Robert G Hamilton, R. Hussain, E. A. Ottesen

Research output: Contribution to journalArticle

Abstract

The immunoradiometric assay (IRMA) for detection of filarial antigens in the serum of patients infected with Brugia malayi (Bm) or the closely related filarial parasite Wuchereria bancrofti (Wb) was investigated, and its performance and clinical utility were examined. Reference sera prepared by the addition of crude Bm antigen (BmA) to negative control human sera provided a reproducible reference curve. The IRMA displayed acceptable precision and reproducibility. Agreement between dilutions (parallelism) was good in sera without specific antibody, but the presence of even modest levels of antibody resulted in nonparallelism in about one-half of the tested sera from endemic areas. Significant reduction in detectable BmA occurred when low levels of specific antibody (

Original languageEnglish (US)
Pages (from-to)2237-2242
Number of pages6
JournalJournal of Immunology
Volume133
Issue number4
Publication statusPublished - 1984

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ASJC Scopus subject areas

  • Immunology

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