Immunological Unresponsiveness to Specific Antigenic Determinants in Human γ G Globulin

Christopher S. Henney, Kimishige Ishizaka

Research output: Contribution to journalArticlepeer-review

Abstract

Immunological unresponsiveness to HGG fragments was induced by intravenous administration of 5 mg soluble antigen. Concomitantly the animals were sensitized to the intact HGG molecule, by injection of 60 μg protein, in complete Freund's adjuvant, into the rear footpads. The immune response was followed at weekly intervals by delayed hypersensitivity measurements, by PCA ti-trations, by gel diffusion precipitin analysis and by radioimmunoelectrophoresis. It was found that when either Fab or Fc fragments were given intravenously, together with an injection of HGG in the footpad, the animal became tolerant to those antigenic determinants represented in the fragments, but was simultaneously sensitized to the other antigenic sites on the molecule. When papain digested HGG (free from undigested material) was administered intravenously, however, antibodies directed against the whole intact molecule, but not against either Fab or Fc fragments, were demonstrable. It would appear that the animals' immune mechanism is capable of subtracting' those determinants shared by both suppressive and sensitizing antigens, from those found in the sensitizing antigen alone.

Original languageEnglish (US)
Pages (from-to)335-341
Number of pages7
JournalProceedings of the Society for Experimental Biology and Medicine
Volume125
Issue number2
DOIs
StatePublished - Jun 1967

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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